Lipid/protein Digestion Quiz Flashcards

1
Q

What is the reaction for lipid digestion?
What is the enzyme used?

A

Triglycerides + H2O -> glycerol + 3 fatty acids
Lipase being the enzyme used to catalyze reaction

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2
Q

Write the reaction for casein precipitation
What was the enzyme?

A

Acidified whole milk -> curds (protein) + whey (Milk fat, and lactose)
Enzyme was rennin

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3
Q

What was the reaction for protein digestion?
What was the enzyme?

A

Casein + H2O -> amino acids
Enzyme was pepsin

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4
Q

What was the reagent for the fatty acid test?

A

Litmus powder

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5
Q

what was the reagent for the amino acid test?

A

Ninhydrin

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6
Q

How do bile salts effect digestion, why?

A

Bile salts allow for emulsification of lipids and water.

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7
Q

How did we isolate the curds from the whey?

A

We strained the curds from the whey using paper towels and light pressure (squeezing)

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8
Q

What did we do to milk to help precipitate occur?

A

-Sawa added buttermilk to acidify it
-We warmed the milk to 37 degrees
- we then added rennin

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9
Q

How did we filter milkshakes after digestion occurred?

A

We used filter paper and a funnel

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10
Q

What occurred in tubes 1-3 of the lipid digestion experiment?

A

1: had just oil + water = no digestion
2: had oil + water + lipase = some digestion
3: had oil + water + lipase + bile salts = most digestion

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11
Q

What colored results were possible within the lipid digestion experiment? what did this mean for the pH?
which tubes had each of the colored results?

A

1: blue = no digestion/ pH remained higher than 7
2: purple = some digestion occurred/ pH decreased slightly
3: red = most digestion occurred/ pH decrease the most

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12
Q

Which was the quantitative/qualitative test for the lipid digestion?

A

Qualitative: viewing change with use of litmus powder
Quantitative: using pH meter to measure pH value

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13
Q

Where is rennin found?

A

In stomach of young mammals

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14
Q

Why did we acidify the milk?

A

To mimic acidic conditions of stomach

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15
Q

What are the molecules found in the milk curds?

A

Casein proteins

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16
Q

What are the molecules found in whey?

A

Milk fats
Lactose carbohydrates

17
Q

What were the color results of the amino test? What did they indicate?

A

Purple - indicated amino acids
Clear - no amino acids