Lipid/protein Digestion Quiz Flashcards
What is the reaction for lipid digestion?
What is the enzyme used?
Triglycerides + H2O -> glycerol + 3 fatty acids
Lipase being the enzyme used to catalyze reaction
Write the reaction for casein precipitation
What was the enzyme?
Acidified whole milk -> curds (protein) + whey (Milk fat, and lactose)
Enzyme was rennin
What was the reaction for protein digestion?
What was the enzyme?
Casein + H2O -> amino acids
Enzyme was pepsin
What was the reagent for the fatty acid test?
Litmus powder
what was the reagent for the amino acid test?
Ninhydrin
How do bile salts effect digestion, why?
Bile salts allow for emulsification of lipids and water.
How did we isolate the curds from the whey?
We strained the curds from the whey using paper towels and light pressure (squeezing)
What did we do to milk to help precipitate occur?
-Sawa added buttermilk to acidify it
-We warmed the milk to 37 degrees
- we then added rennin
How did we filter milkshakes after digestion occurred?
We used filter paper and a funnel
What occurred in tubes 1-3 of the lipid digestion experiment?
1: had just oil + water = no digestion
2: had oil + water + lipase = some digestion
3: had oil + water + lipase + bile salts = most digestion
What colored results were possible within the lipid digestion experiment? what did this mean for the pH?
which tubes had each of the colored results?
1: blue = no digestion/ pH remained higher than 7
2: purple = some digestion occurred/ pH decreased slightly
3: red = most digestion occurred/ pH decrease the most
Which was the quantitative/qualitative test for the lipid digestion?
Qualitative: viewing change with use of litmus powder
Quantitative: using pH meter to measure pH value
Where is rennin found?
In stomach of young mammals
Why did we acidify the milk?
To mimic acidic conditions of stomach
What are the molecules found in the milk curds?
Casein proteins
