Enzymes and Carbohydrate Digestion quiz Flashcards
Describe in general how enzymes work
they use their active cites to convert substrate into products
what are the two types of reactions that can occur using enzymes?
anabolic reactions
catabolic reactions
what is an anabolic reaction?
it uses energy to build large molecules from small pieces
what is a catabolic reaction?
it produces energy by breaking down large molecules into small pieces
how do enzymes function in a hydrolysis reaction?
they catalyze catabolic reactions
what are examples of conditions that denature proteins?
boil them
change pH
high salt
what condition did we use to denature the enzymes within our experiment?
boiled them
Write the enzymatic reaction for starch digestion
how did we test for starch? what reagent, what color left a positive/negative result?
reagent: Lugol’s iodine
positive: black = starch is present
negative: brown = no starch
how did we test for sugar? what reagent, what color left a positive/negative result?
reagent: Benedict’s reagent
positive: orange = sugar is present
negative: blue = no sugar
there are two test tubes with enzymes, one is boiled, one is left untouched. which will be denatured?
the boiled one
what color would an unboiled test tube containing amylase and starch be when added Lugol’s iodine? what does this test for?
brown; negative for presence of starch
what color would a bolied test tube containing amylase and starch be when Benedict’s reagent is added? what does this test for?
blue; negative for presence of sugar
what color would a boiled test tube containing amylase and starch be when Lugol’s iodine is added? what does this test for?
black; positive for presence of starch
what color would an unbolied test tube containing amylase and starch be when Benedict’s reagent is added? what does this test for?
orange; positive for presence of sugar
