Lab quiz 1

Question 1

what lymphocyte? Describe it’s traits.

Answer 1

Neutrophil
- granules
- multi-lobed nucleus

Question 2

what lymphocyte? Describe it’s traits.

Answer 2

Neutrophil
- granules
- multi-lobed nucleus

Question 3

Answer 3

eosinophil
- bilobed nucleus
- very red granules

Question 4

Answer 4

Neutrophil
- granules
- multi-lobed nucleus

Question 5

Answer 5

Basophil
- bilobed nucleus
- purplish black granules (overwhelm cell/ make it hard to see nucleus)

Question 6

Answer 6

lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES

Question 7

Answer 7

monocyte
- kidney shaped nucleus (puffy)
- larger than RBCs
- abundant pale blue cytoplasm
NO GRANULES

Question 8

Answer 8

lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES

Question 9

Answer 9

monocyte
- kidney shaped nucleus (puffy)
- larger than RBCs
- abundant pale blue cytoplasm
NO GRANULES

Question 10

Answer 10

lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES

Question 11

Answer 11

eosinophil
- bilobed nucleus
- very red granules

Question 12

what is the name and what did we do for the first lab experiment?

Answer 12

Total RBC count
-diluted blood sample
- counted all RBCs in the 5x5 central grid of hemocytometer to obtain a raw number
- multiplied raw number with dilution factor to obtain RBC concentration

Question 13

what does the area of the 5x5 grid of the hemocytometer measure?

Answer 13

1 microliter

Question 14

what ratio was the RBC blood sample we used diluted to?

Answer 14

1:10,000

Question 15

what magnification did we use for the first experiment?

Answer 15

10x for a total magnification of 100x

Question 16

what is the equation for finding the actual total concentration of RBC per microliter?

Answer 16

raw data (number counted) x dilution factor (10,000 in this case)
= concentration of RBC per microliter

Question 17

If the raw data of RBCs counted was 459, what is the actual concentration?

Answer 17

459 x 10,000
= 4,590,000 RBC/microliter

Question 18

what is the purpose of the first experiment?

Answer 18

determines concentration of RBC in a sample

Question 19

what is the name of the slide we use to count RBCs?

Answer 19

hemocytometer

Question 20

what is the normal range for RBC count?

Answer 20

4,000,000 RBC/microliter - 6,000,000 RBC/microliter

Question 21

If Khloe’s RBC count is 3,600,000 RBC/microliter, what blood disorder would she have?

Answer 21

anemia

Question 22

How do we determine a quantitative amount?

Answer 22

precise amount found using special tool

Question 23

How do we determine a qualitative amount?

Answer 23

determine relative amount using best judgement

Question 24

If Kim’s RBC count is 8,000,000 RBC/microliter, what blood disorder would she have?

Answer 24

polycythemia - too many RBC in blood
poly- many
cyte - cell
hemia - in the blood

Question 25

How is the first experiment useful in the medical field?

Answer 25

to determine blood disorders

Question 26

what is the name of the second experiment and how did we preform it?

Answer 26

Differential WBC count
- scan through slide and I.D. each WBC you see
- keep tally until around 100 cells
- calculate percentage (# of class / total # of cells)

Question 27

what is the purpose of the Differential WBC count experiment and why might we use it?

Answer 27

count all the various types of leukocytes to see if there are any overabundance of any one leukocyte which would diagnose Leukemia

Question 28

what do neutrophil, eosinophil, and basophil have in common?

Answer 28

they are all granulocytes (contain granules)

Question 29

what do lymphocyte and monocyte have in common?

Answer 29

they are all agranulocytes (do not contain granules)

Question 30

Briefly describe how we can identify a neutrophil and the percentage they are found in

Answer 30

have trilobed nucleus and tiny red granules (50-70%)

Question 31

Briefly describe how we can identify a eosinophil and the percentage they are found in

Answer 31

have bilobed nucleus and large red granules (2-4%)

Question 32

Briefly describe how we can identify a basophil and the percentage they are found in

Answer 32

have U-shaped nucleus and large dark granules (<1%)

Question 33

Briefly describe how we can identify a lymphocytes and the percentage they are found in

Answer 33

smallest with large, round nucleus and small rim of cytoplasm (very similar size to RBC) (20-40%)

Question 34

Briefly describe how we can identify a monocyte and the percentage they are found in

Answer 34

monstrously large with puffy U-shaped nucleus (3-8%)

Question 35

Describe two ways to differentiate between an eosinophil and a neutrophil in the microscope.

Answer 35

1.) neutrophil have three lobes while eosinophil have 2 lobes
2.) Neutrophil have small red granules while eosinophil have large red granules

Question 36

If Khloe had 58 neutrophil and 109 total leukocytes where counted, what percentage of neutrophil does she have? Is this normal?

Answer 36

( 58 / 109 ) 100
= 53%

Question 37

Why would basophils and lymphocytes be confused?

Answer 37

If you are only looking at the nucleus of a lymphocyte, the nucleus can look like the dark granular structure of a whole basophil

Question 38

name the qualitative experiment we did

Answer 38

Differential WBC count