Lab quiz 1 Flashcards
what lymphocyte? Describe it’s traits.
Neutrophil
- granules
- multi-lobed nucleus
what lymphocyte? Describe it’s traits.
Neutrophil
- granules
- multi-lobed nucleus
eosinophil
- bilobed nucleus
- very red granules
Neutrophil
- granules
- multi-lobed nucleus
Basophil
- bilobed nucleus
- purplish black granules (overwhelm cell/ make it hard to see nucleus)
lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES
monocyte
- kidney shaped nucleus (puffy)
- larger than RBCs
- abundant pale blue cytoplasm
NO GRANULES
lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES
monocyte
- kidney shaped nucleus (puffy)
- larger than RBCs
- abundant pale blue cytoplasm
NO GRANULES
lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES
eosinophil
- bilobed nucleus
- very red granules
what is the name and what did we do for the first lab experiment?
Total RBC count
-diluted blood sample
- counted all RBCs in the 5x5 central grid of hemocytometer to obtain a raw number
- multiplied raw number with dilution factor to obtain RBC concentration
what does the area of the 5x5 grid of the hemocytometer measure?
1 microliter
what ratio was the RBC blood sample we used diluted to?
1:10,000
what magnification did we use for the first experiment?
10x for a total magnification of 100x
what is the equation for finding the actual total concentration of RBC per microliter?
raw data (number counted) x dilution factor (10,000 in this case)
= concentration of RBC per microliter
If the raw data of RBCs counted was 459, what is the actual concentration?
459 x 10,000
= 4,590,000 RBC/microliter
what is the purpose of the first experiment?
determines concentration of RBC in a sample
what is the name of the slide we use to count RBCs?
hemocytometer
what is the normal range for RBC count?
4,000,000 RBC/microliter - 6,000,000 RBC/microliter
If Khloe’s RBC count is 3,600,000 RBC/microliter, what blood disorder would she have?
anemia
How do we determine a quantitative amount?
precise amount found using special tool
How do we determine a qualitative amount?
determine relative amount using best judgement
If Kim’s RBC count is 8,000,000 RBC/microliter, what blood disorder would she have?
polycythemia - too many RBC in blood
poly- many
cyte - cell
hemia - in the blood