Lab quiz 1 Flashcards
what lymphocyte? Describe it’s traits.
Neutrophil
- granules
- multi-lobed nucleus
what lymphocyte? Describe it’s traits.
Neutrophil
- granules
- multi-lobed nucleus
eosinophil
- bilobed nucleus
- very red granules
Neutrophil
- granules
- multi-lobed nucleus
Basophil
- bilobed nucleus
- purplish black granules (overwhelm cell/ make it hard to see nucleus)
lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES
monocyte
- kidney shaped nucleus (puffy)
- larger than RBCs
- abundant pale blue cytoplasm
NO GRANULES
lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES
monocyte
- kidney shaped nucleus (puffy)
- larger than RBCs
- abundant pale blue cytoplasm
NO GRANULES
lymphocyte
- small (similar size to RBC)
- large nucleus with small thin blue rim of cytoplasm
NO GRANULES
eosinophil
- bilobed nucleus
- very red granules
what is the name and what did we do for the first lab experiment?
Total RBC count
-diluted blood sample
- counted all RBCs in the 5x5 central grid of hemocytometer to obtain a raw number
- multiplied raw number with dilution factor to obtain RBC concentration
what does the area of the 5x5 grid of the hemocytometer measure?
1 microliter
what ratio was the RBC blood sample we used diluted to?
1:10,000
what magnification did we use for the first experiment?
10x for a total magnification of 100x
what is the equation for finding the actual total concentration of RBC per microliter?
raw data (number counted) x dilution factor (10,000 in this case)
= concentration of RBC per microliter
If the raw data of RBCs counted was 459, what is the actual concentration?
459 x 10,000
= 4,590,000 RBC/microliter
what is the purpose of the first experiment?
determines concentration of RBC in a sample
what is the name of the slide we use to count RBCs?
hemocytometer
what is the normal range for RBC count?
4,000,000 RBC/microliter - 6,000,000 RBC/microliter
If Khloe’s RBC count is 3,600,000 RBC/microliter, what blood disorder would she have?
anemia
How do we determine a quantitative amount?
precise amount found using special tool
How do we determine a qualitative amount?
determine relative amount using best judgement
If Kim’s RBC count is 8,000,000 RBC/microliter, what blood disorder would she have?
polycythemia - too many RBC in blood
poly- many
cyte - cell
hemia - in the blood
How is the first experiment useful in the medical field?
to determine blood disorders
what is the name of the second experiment and how did we preform it?
Differential WBC count
- scan through slide and I.D. each WBC you see
- keep tally until around 100 cells
- calculate percentage (# of class / total # of cells)
what is the purpose of the Differential WBC count experiment and why might we use it?
count all the various types of leukocytes to see if there are any overabundance of any one leukocyte which would diagnose Leukemia
what do neutrophil, eosinophil, and basophil have in common?
they are all granulocytes (contain granules)
what do lymphocyte and monocyte have in common?
they are all agranulocytes (do not contain granules)
Briefly describe how we can identify a neutrophil and the percentage they are found in
have trilobed nucleus and tiny red granules (50-70%)
Briefly describe how we can identify a eosinophil and the percentage they are found in
have bilobed nucleus and large red granules (2-4%)
Briefly describe how we can identify a basophil and the percentage they are found in
have U-shaped nucleus and large dark granules (<1%)
Briefly describe how we can identify a lymphocytes and the percentage they are found in
smallest with large, round nucleus and small rim of cytoplasm (very similar size to RBC) (20-40%)
Briefly describe how we can identify a monocyte and the percentage they are found in
monstrously large with puffy U-shaped nucleus (3-8%)
Describe two ways to differentiate between an eosinophil and a neutrophil in the microscope.
1.) neutrophil have three lobes while eosinophil have 2 lobes
2.) Neutrophil have small red granules while eosinophil have large red granules
If Khloe had 58 neutrophil and 109 total leukocytes where counted, what percentage of neutrophil does she have? Is this normal?
( 58 / 109 ) 100
= 53%
Why would basophils and lymphocytes be confused?
If you are only looking at the nucleus of a lymphocyte, the nucleus can look like the dark granular structure of a whole basophil
name the qualitative experiment we did
Differential WBC count
