lipid metabolism II Flashcards
what lipases are involved in the breakdown of TAGs
ATGL - adipose triglyceride lipase
hormone sensitive lipase (HSL)
lipoprotin lipase (LPL)
monoacylglycerol lipase (MAG Lipase)
transport of Fatty Acids:
short chain fatty acids ____
long chain complexed with ____ for transport
soluble
albumin
breakdown of TAGs
fat cell:
TAG → ____ and ____ ____
liver cell:
glycerol → ____ → ____
glycerol → ____ → ____
other tissue:
fatty acids → ____ ____ ____ → ____ ____ → TCA cycle
fat cell:
glycerol and fatty acids
liver cell:
glycolysis → pyruvate
gluconeogenesis → glucose
other tissues:
fatty acid oxidation → acetyl CoA
activation of HSL is modulated by ____
phosphorylation
____ and ____ are 2 major signals that promote mobilization of TAGs
hunger and exercise
major controllers of HSL signaling in hunger and exercise:
____ (secreted in response to hunger)
____ (secreted in resopnse to exercise)
both of these phosphorylate HSL (activate it) and promote ____ in ____
glucagon
epinephrine
lipolysis in adipocytes
mechanisms of regulation of HSL:
____ status signal inhibits mobilization of TAGs
____ (secreted in resopnse to high carb meal)
____ HSL (via activation of protein phosphatase I) to inhibit lipolysis
fed
insulin
dephosphorylates
perilipin:
- family of proteins that coat ____ ____ in ____ and muscle cells
- regulate ____ by controlling physical access to ____ (lipid breakdown enzyme)
- perilipins regulated by ____ (phosphorylation allows association with HSL) which promotes lipolysis
- overexpression of Perilipin I ____ lipolysis
- target of ____ treatment
- lipid droplets adipocytes
- lipolysis HSL
- PKA
- inhibits
- obesity
fatty acid breakdownA: an overview:
phase I:
phase II:
phase I: activation and transport to mitochondiral matrix
phase II: beta oxidation
TAGs → diacylglycerol (via ____ ) → monoacylglycerol (via ____ ) → glycerol (via ____ )
HSL
lipoprotein lipase
MAG
activators of HSL:
inhibitors of HSL:
glucagon, epinephrine, norepinephrine,
insulin
mobilization of FAs from Adipocytes: hunger and exercise
- glucagon and/or epinephrine bind to ____
- ____ is formed
- cAMP activates ____
- PKA phosphorylates ____ and ___
- glucagon and epinephrine also release another activator that activates ____
- GPCR
- cAMP
- PKA
- perilipin and HSL
- ATGL
mobilization of FAs from Adipocytes: Fed
- ____ binds to RTK (receptor tyrosin kinase)
- RTK activates ____
- protein phosphatase 1 (PP1) dephosphorylates and inactivates ____
- insulin
- protein phosphatase 1
- HSL
phase I FA breakdown:
- as long-chain FA-albumin complex crosses the plasma membrane, it loses ____
- outer mitochondrial membrnae is not permeable to ____ FAs
- must first be combined with ____ to make fatty acyl CoA in cytosol
- fatty acyl CoA has now crossed the outer mitochondrial membrane and is present in the ____ space
- fatty acyl CoA cannot cross ____ mitochondrial membrane
- ____ must be added which replaces CoA
- ____ is now permeable across the inner mitochondrial membrane
- once in the mitochondrial matrix, ____ is replaced with ____ to form fatty acyl CoA
- fatty acyl CoA becomes substrate for ____
- albumin
- free
- CoA
- intermembrane
- inner
- carnitine
- fatty acyl carnitine
- carnatine CoA
- beta-oxidation
translocation to mitochondrial matrix:
- carnitine → acyl carnitine via ____ ____ ____
- acylecarnitine translocates across inner mitochondrial membrane via ____
- acyl carnitine → carnitine via ____ ____ ____
- carnitine acyltransferase I
- translocase
- carnitine acyltransferase II