Lipase

Question 1

Other name for Lipase

Answer 1

Triacyl glycerol acylhydrolase

Question 2

Action of Lipase

Answer 2

Hydrolysis of ester bond producing 2 monoglyceride + 2 fatty acid

Question 3

Lipase is a specific enzyme marker for what conditon

Answer 3

Acute pancreatitis

Question 4

lipase is not involved in any ____

Answer 4

renal disorders (too large too be filtered)

Question 5

Earliest enzyme to be elevated in acute pancreatitis

Answer 5

Amylase

Question 6

Rise, Peak, Retention of Lipase in Acute Pancreatitis

Answer 6

Rise: 6 hours
Peak: 24 hours
Normalize: 7 days

Question 7

Soluble form of triglyceride is being absorbed by

Answer 7

Small intestine (microvilli)

Question 8

Enzymatic Assay used in Lipase

Answer 8

Cherry Krandall
Tietz

Question 9

Substrate used in Enzymatic Method

Answer 9

50% olive oil
Triolein

Question 10

Titration agent for Cherry Krandall and tietz

Answer 10

0.4 N NaOH

Question 11

Indicator used in Cherry Krandall

Answer 11

Phenolpthalein

Question 12

Indicator used in Tietz

Answer 12

Thymolphthalein + Veronal

Question 13

Product of Enzymatic Assay Lipase

Answer 13

Fatty Acids (Oleic acid)

Question 14

Cherry krandall produces what color

Answer 14

Pink

Question 15

Tietz produces what color

Answer 15

Blue

Question 16

Turbidimetry method:

Increased turbidity; ______ absorbance

Answer 16

Increase absorbance

Question 17

There is a decreased turbidity due to

Answer 17

Triglyceride from turbid suspension is converted to fatty acid (more soluble than triglyceride)

Question 18

Condition by which should be avoided when measuring Lipase

Answer 18

Hemolysis (FALSE DECREASED)