Lactate Dehydrogenase (LDH) Flashcards
1
Q
Catalyzes interconversion of lactic and pyruvic
A
Lactate Dehydrogenase
2
Q
Major Sources of LDH
A
Heart
Liver
Skeletal muscle
RBC
3
Q
LDH subunits
A
Heart
Muscle
4
Q
Coenzyme of LDH
A
NAD
5
Q
Enzymatic Assay:
Forward Reaction
A
Wacker Method
6
Q
Wacker Method:
Product and Absorbance
A
Reduced NAD (NADH + H)
@340 nm
7
Q
Wacker Method:
pH
A
8.3-8.9
8
Q
Enzymetic Assay of LDH:
Reverse Reaction
A
Wroleubski La Due
9
Q
Wrobleuski La Due:
Product and Absorbance
A
NAD
Decrease Absorbance @340nm
10
Q
Wrobleubski La Deu:
pH
A
7.1-7.4
11
Q
greater affinity with H subunit
A
a-HBD (a-hydroxybutyrate dehydrogenase)
12
Q
LDH-1 monomers
A
HHHH
13
Q
LDH 1 Clinical Significance
A
MI
Hemolytic Anemia
14
Q
LDH 1 associate organ
A
Heart, RBC
15
Q
LDH 2 monomers
A
HHHM