Lactate Dehydrogenase (LDH) Flashcards by FRANCHEZKA MARIE IGNACIO

Q

Catalyzes interconversion of lactic and pyruvic

A

Lactate Dehydrogenase

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Q

Major Sources of LDH

A

Heart
Liver
Skeletal muscle
RBC

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Q

LDH subunits

A

Heart
Muscle

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Q

Coenzyme of LDH

A

NAD

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Q

Enzymatic Assay:

Forward Reaction

A

Wacker Method

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Q

Wacker Method:

Product and Absorbance

A

Reduced NAD (NADH + H)
@340 nm

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Q

Wacker Method:

pH

A

8.3-8.9

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Q

Enzymetic Assay of LDH:

Reverse Reaction

A

Wroleubski La Due

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Q

Wrobleuski La Due:

Product and Absorbance

A

NAD
Decrease Absorbance @340nm

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Q

Wrobleubski La Deu:

pH

A

7.1-7.4

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Q

greater affinity with H subunit

A

a-HBD (a-hydroxybutyrate dehydrogenase)

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Q

LDH-1 monomers

A

HHHH

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Q

LDH 1 Clinical Significance

A

MI
Hemolytic Anemia

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Q

LDH 1 associate organ

A

Heart, RBC

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Q

LDH 2 monomers

A

HHHM

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Q

LDH 2 associated organs

A

Heart
RBC

Q

LDH 2 Clinical Significance

A

Radial infarction
Megaloblastic Anemia

Q

LDH 3 associated organs

A

Lungs
Spleen
Pancrease

Q

LDH 3 Clinical Significance

A

Pulmonary embolism

Q

LDH 4 associated organs

A

Liver

Q

LDH 4 clinical significance

A

hepatic injury

Q

LDH 5 associated organs

A

Skeletal muscle

Q

LDH 5 Clinical significance

A

Skeletal muscle injury

Q

Relative concentration of LDH in serum

A

2>1>3>4>5

Q

LDH concentration if patient has AMI or intravascular hemolysis

A

1>2>3>4>5 (FLIPPED PATTERN)

Q

LDH level in AMI: Rise, Peak, Remains elevated

A

Rise: 10-24 hrs
Peak: 48-72
Elevated: 10 days

Q

Reference range of LD

A

100-225 U/L

Q

Is LDH fastest or slowest to elevate in AMI among AMI markers

A

SLOWEST