Amylase (AMY)

Question 1

other name for amylase

Answer 1

Alpha 1,4-glucan-4-glucohydrolase

Question 2

function of amylase

Answer 2

• digestion and absorption of carbohydrates
• breakdown of branching linkages of polysaccharides

Question 3

breakdown of starch and glycogen via

Answer 3

alpha, 1-6 branching linkages

Question 4

major tissues involved in amylase

Answer 4

pancreas
salivary gland

Question 5

minor tissues involved in amylase

Answer 5

Fallopian tube
Adipose tissue
Skeletal muscle
Small Intestine

FASS

Question 6

Clinical Significance of Amylase

Answer 6

Acute pancreatitis
Renal Failture
Parotitis
Mumps

Question 7

Most frequent in male; extreme pain while sleeping

Answer 7

Acute pancreatitis

Question 8

fastest to elevate in acute pancreatitis

Answer 8

Amylase

Question 9

Rise of amylase after onset of attack

Answer 9

2-12 hrs

Question 10

Peak of Amylase (AP)

Answer 10

24 Hrs

Question 11

Incase of AP it Normalize Amylase within

Answer 11

3-4 days

Question 12

is amylase normal in urine

Answer 12

yes

Question 13

smallest enzyme present in plasma

Answer 13

amylase

Question 14

Relationship between levels of amylase in plasma and urine

Answer 14

Directly proportional

Question 15

Amylase level: Renal Disease

Answer 15

Increase in Plasma
Decrease in Urine

Question 16

Amylase level: Acute Pancreatitis

Answer 16

Increase in Plasma & Urine

Question 17

Isoenzymes of Amylase

Answer 17

Salivary Amylase (S Type)
Pancreatic Amylase (P Type)

Question 18

Salivary Amylase Characteristics

Answer 18

Ptyalin
Fast moving

Question 19

Pancreatic Amylase Characteritics

Answer 19

Amylopsin
Slow moving

Question 20

Amylase Methodologies

Answer 20

Amyloclastic
Saccharogenic
Chromogenic
Continuous Monitoring

SACC

Question 21

Coupling of several enzyme to measure enzyme activity

Answer 21

Continuous monitoring

Question 22

measures the disappearance of starch substance

Answer 22

Amyloclastic

Question 23

measures the appearance of substance (starch —> reducing sugar)

Answer 23

Saccharogenic

Question 24

measures increasing color from product
(Insoluble starch dye - soluble starch dye)

Answer 24

Chromogenic

Question 25

Iodine reacts only with

Answer 25

Complex sugars

Question 26

Amyloclastic: Once the enzyme attacks the substrate it will result to

Answer 26

decrease substrate concentration

Question 27

Amyloclastic: If the substrate concentration decreases what will happen next

Answer 27

Starch releases simple sugars = Iodine will not react with it = Decrease color intensity

Question 28

Amyloclastic: Substitute for Starch as substrate

Answer 28

Glycogen

Question 29

If glycogen will react to iodine it will produce

Answer 29

Mahogany brown color

Question 30

Saccharogenic: once the amylase attacks starch, starch will release

Answer 30

reducing sugar

Question 31

Saccharogenic: Reducing sugar from Starch has what kind of relationship to enzyme activity

Answer 31

Directly proportional

Question 32

Chromogenic: Insoluble starch dye will produce fragment, fragment is responsible for

Answer 32

Color reaction

Question 33

Chromogenic: product produced has what kind of relationship to enzyme activity

Answer 33

Directly Proportional

Question 34

Continuous Monitoring Assay:

Last enzyme involved in reaction

Answer 34

Oxidoreductase

Question 35

Continuous Monitoring Assay:

Last reaction

Answer 35

conversion of coenzyme (NAD –> NADH)

Question 36

Continuous Monitoring Assay:

Measures absorbance at?

Answer 36

Increase @340 nm

Question 37

Continuous Monitoring Assay:

Second substrate, indicator used

Answer 37

A-glucosidase
Hexokinase
G-6-PD