Creatine Kinase (CK)

Question 1

storage of high-energy creatine phosphate in muscle

Answer 1

Creatine kinase

Question 2

high energy resorvoir for muscles

Answer 2

Creatine phosphate

Question 3

Creatine kinase is widely distributed; high activities in

Answer 3

Skeletal muscle, Brain, Heart

Question 4

Creatine kinase structure

Answer 4

dimeric enzyme

Question 5

2 monomer subunits of CK

Answer 5

Brain
Muscle

Question 6

Enzymatic Reaction of CK

Answer 6

Creatine + ATP –> Creatine phosphate + ADP

Question 7

CK-3 (MM)

Answer 7

Muscle Type

Question 8

Slowest to migrate towards anode

Answer 8

CK-3 Muscle Type

Question 9

CK-3 is found in

Answer 9

Striated muscles
Healthy individuals

Question 10

CK-2 (MB)

Answer 10

Hybrid Type

Question 11

Second fastest to migrate towards anode

Answer 11

CK-2 (MB)

Question 12

CK-2 (MB) is found in

Answer 12

Heart tissue
Cardiac muscles

Question 13

CK-1 (BB)

Answer 13

Brain Type

Question 14

CK-1 is found in

Answer 14

CNS
GI tract
Uterus

Question 15

type of CK isoenzyme that is least found in healthy individuals

Answer 15

CK-1 (BB)

Question 16

Fastest to migrate towards anode

Answer 16

CK-1 (BB)

Question 17

Improves test specificity;

Answer 17

Isoenzyme measurement

Question 18

Total CK

Answer 18

CK-1, CK-2, CK-3

Question 19

After MI, CK-MB values:

Rise, Peak, Normalize

Answer 19

Rise: 4-8 hrs
Peak: 12-24 hrs
Normalize: 48 hrs-72 hrs (3 days)

Question 20

Among AMI markers, CK-MB is the last enzyme to elevate

True or false

Answer 20

False (CK-MB IS THE FIRST ENZYME TO ELEVATE IN AMI)

Question 21

first PROTEIN to elevate upon onset of AMI

Answer 21

Myoglobin (faster than CK-MB)

Question 22

Reference value of CK-MM

Answer 22

94%-98%

Question 23

Reference value of CK-MB

Answer 23

2%-6%

Question 24

Increased CK-BB indicates

Answer 24

CSF leakage; Blood Brain Barrier damage

Question 25

Other CK isoenzyme that is with Antibodies/lipoprotein

Answer 25

Macro-CK

Question 26

Macro-CK with Antibodies

Answer 26

CK-BB

Question 27

Antibodies found in Macro CK-BB

Answer 27

IgG
IgA

Question 28

Lipoprotein is found in what isoenzyme

Answer 28

CK-MM

Question 29

Isoenzyme of CK that migrates towards CATHODE

Answer 29

Mitochondrial CK

Question 30

Methods of Determination for CK

Answer 30

Forward Reaction (Tanzer-Gilvarg)
Reverse Reaction (Oliver Rosalki)

Question 31

Forward Reaction (Tanzer Gilvarg):

pH

Answer 31

9.0

Question 32

Forward Reaction (Tanzer Gilvarg):

Product and absorbance

Answer 32

Oxidized (NAD)

Decrease absorbance @340 nm

Question 33

Forward Reaction (Tanzer Gilvarg):

Reagent

Answer 33

PK (Pyruvate Kinase)
Lactate Dehydrogenase

Question 34

Forward Reaction (Tanzer Gilvarg):

PK Reaction

Answer 34

ADP + phosphoenulpyruvate —–> Pyruvate + ATP

Question 35

Forward Reaction (Tanzer Gilvarg):

Lactate Dehydrogenase Reaction

Answer 35

Pyruvate + NADH + H —> Lactate + NAD

Question 36

Reverse Reaction for CK

Answer 36

Oliver Rosalki

Question 37

Reverse Reaction CK:

pH

Answer 37

6.8 acidic

Question 38

Reverse Reaction (Oliver Rosalki):

Product and absorbance

Answer 38

Reduced: NADH + H
; Increased Absorbance @340 nm

Question 39

Reverse Reaction (Oliver Rosalki):

Reagent

Answer 39

Hexokinase
G-6-PD

Question 40

Reverse Reaction (Oliver Rosalki):

Hexokinase Reaction

Answer 40

ATP + Glucose —> Glucose-6-phosphate + ADP

Question 41

Reverse Reaction (Oliver Rosalki):

G-6-PD Reaction

Answer 41

Glucose-6-phosphate + NADP –> 6-phosphogluconate + NADPH + H

Question 42

CK: Source of Error

Answer 42

Hemolysis (false increased)
Exposure to light (false decreased)
Physical Activity and IM injections (falsely increased)

Question 43

A type of kinase enzyme that is released once blood is hemolyzed; causing false elevation of CK

Answer 43

Adenylate Kinase

Question 44

Reference Value of CK:

Male

Answer 44

15-160 U/L