Aspartate Amino Transferase (AST)

Question 1

Other name for AST

Answer 1

SGOT
Serum Glutamic Oxaloacetic Transaminase

Question 2

transfer amino acid to aspartate and a-ketoacid

Answer 2

AST

Question 3

Product of AST

Answer 3

Glutamate and Oxaloacetate

Question 4

AST is responsible for

Answer 4

Synthesis and degradation of amino acid

Question 5

True or False

AST is liver specific

Answer 5

FALSE (not liver specific)

Question 6

AST is found in

Answer 6

Cardiac
Skeletal Muscle
Liver

Question 7

2 Isoenzymes of AST

Answer 7

Cytoplasmic AST - predominant isoenzyme in serum
Mitochondrial AST

Question 8

Reaction of AST

Answer 8

Aspartate + a-ketoglutarate -> oxaloacetate
+ glutamate

Question 9

Clinical Significance of AST

Answer 9

Acute MI
Chronic Disorder of liver
Skeletal muscle diseasetransamina

Question 10

Acute MI levels: Rise, Peak, Normal

Answer 10

Rise: 6-8 hrs
Peak: 24 hrs
Normalize: 5 days

Question 11

AST is also increased or decreased in Hepatocellular and skeletal disorder

Answer 11

Increased

Question 12

Enzymatic Assay for AST

Answer 12

Karmen’s Method

Question 13

Karmen’s Method measures AST based on

Answer 13

Decreased Absorbance @340 nm (NAD)

Question 14

secondary substrate, coupling, indicator used in Karmen’s Method

Answer 14

Malate dehydrogenase

Question 15

AST is falsely DECREASED/INCREASED in hemolysed sample

Answer 15

Falsely increased

Question 16

Reaction of AST when Malate Dehydrogenase is added

Answer 16

aspartate + a-ketoglutarate = oxaloacetate + glutamate

Oxaloacetate + NADH + H (Malate dehy) = malate + NAD