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MTAP 2 FINALS - CC2 > Alkaline Phosphatase (ALP) > Flashcards

Alkaline Phosphatase (ALP) Flashcards

1
Q

Action of ALP

A

Liberate inorganic phosphate from organic phosphate

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2
Q

Liberation of inorganic phosphate from organic phosphate produce

A

Alcohol

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3
Q

ALP catalyze

A

Hydrolysis of phosphomonoester at an alkaline pH (9-10)

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4
Q

Activator used by ALP

A

Magnesium

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5
Q

In healthy individuals, ALP predominantly found in

A

Liver
Bones (osteoblast)

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6
Q

Major sources of ALP

A

Liver
Bones
Placenta
Kidney
Intestine

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7
Q

Enzymatic Assay used in ALP

A

Bowers and Mccomb

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8
Q

What is being measured in Bowers and Mccomb

A

P-nitrophenol

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9
Q

Color of P-nitrophenol

A

Yellow

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10
Q

Absorbance used in measuring ALP

A

405 nm

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11
Q

Uses B-glycero-phosphate as substrate

A

Bodansky
Shinowara
Jones
Reinhart

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12
Q

Product of Bodansky, Shinowara, Jones, and Reinhart

A

Inorganic phosphate + glycerol

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13
Q

A method that uses P-nitrophenyl phosphate as substrate

A

Bessy, Lowry, and Brock
Bowers and Mccomb

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14
Q

Method that used phenyl phosphate as substrate

A

King and Armstrong

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15
Q

Product of King and Armstrong Reaction

A

Phenol

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16
Q

Reference range of ALP:

Adult

A

30-90 U/l

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17
Q

Reference range of ALP:

0-3 months

A

70-220 U/l

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18
Q

Reference range of ALP:

3-10 years

A

50-260 U/l

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19
Q

Reference range of ALP:

10 yrs-puberty

A

60-295 U/l

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20
Q

why is it ALP is increased in ages 3-puberty

A

Osteoblast Activity is Active

21
Q

What is used to differentiate ALP isoenzyme

A

Electrophoresis

22
Q

ALP Electrophoretic Migration:

Fastest to slowest

A

Love, BPI

Liver - Bone - Placenta - Intestine

23
Q

ALP isoenzyme:

Fastest to migrate towards anode

A

Liver ALP

24
Q

Liver ALP is consist of what factors

A

Major Type
Fast Liver

25
Q

Heat labile fraction of ALP isoenzyme

A

Bone ALP

26
Q

Bone ALP:

temperature increases, ALP ____

A

Decreases

27
Q

Bone ALP is significantly increased in what conditions

A

Paget’s disease
Ostitis deformans

28
Q

Clinical Significance of Bone ALP

A

Bone disease
Healing of bone fracture
Physiologic bone growth

29
Q

ALP isoenzyme:

Heat stable fraction

A

Placental ALP

30
Q

Placental ALP is abundant at what stage of gestation

A

16th - 20th week of gestation

31
Q

Placental ALP is significant in what condition

A

carcinoplacental ALP

32
Q

Intestinal ALP is physiologically increased in what condition

A

After consumption of fatty meal (Blood type B and O)

33
Q

Intestinal ALP is pathologically increased in

A

GIT disorders

34
Q

Reagent that inhibits Placental and Intestinal ALP

A

Phenylalanine

35
Q

Reagent that inhibits Bone ALP

A

3 M urea

36
Q

Reagent that inhibits Bone and Liver ALP

A

Levamisole

37
Q

Test used to differentiate the elevation of Liver or Bone ALP

A

Heat stability Test

38
Q

Heat stabitility Characteristics of ALP

A

P>I>L>B

39
Q

Residual Activity decreased to >20%

A

Liver ALP

40
Q

Residual activity decreased to <20%

A

Bone ALP (heat labile)

41
Q

Heat stability test is done by

A

Heating serum @56 deg cel. For 10 mins

42
Q

Carcino Placental ALP are

A

Regan ALP
Nagao ALP

43
Q

Most heat stable Placental ALP fraction

A

Regan ALP

44
Q

Regan ALP is found in

A

Lungs
Breast
Gynecological disorder
Co-migrator of Bone

45
Q

Regan ALP can still act @what temp

A

65 deg cel. For 30 minutes

46
Q

Placental ALP fraction found in

Adenocarcinoma of pancreas
Bile duct
Pleural Cancer

A

Nagao ALP

47
Q

Nagao ALP inhibitor

A

Phenylalanine and L-leucine

48
Q

Regan ALP inhibitor

A

Phenylalanine

MTAP 2 FINALS - CC2 (11 decks)

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