Alkaline Phosphatase (ALP)

Question 1

Action of ALP

Answer 1

Liberate inorganic phosphate from organic phosphate

Question 2

Liberation of inorganic phosphate from organic phosphate produce

Answer 2

Alcohol

Question 3

ALP catalyze

Answer 3

Hydrolysis of phosphomonoester at an alkaline pH (9-10)

Question 4

Activator used by ALP

Answer 4

Magnesium

Question 5

In healthy individuals, ALP predominantly found in

Answer 5

Liver
Bones (osteoblast)

Question 6

Major sources of ALP

Answer 6

Liver
Bones
Placenta
Kidney
Intestine

Question 7

Enzymatic Assay used in ALP

Answer 7

Bowers and Mccomb

Question 8

What is being measured in Bowers and Mccomb

Answer 8

P-nitrophenol

Question 9

Color of P-nitrophenol

Answer 9

Yellow

Question 10

Absorbance used in measuring ALP

Answer 10

405 nm

Question 11

Uses B-glycero-phosphate as substrate

Answer 11

Bodansky
Shinowara
Jones
Reinhart

Question 12

Product of Bodansky, Shinowara, Jones, and Reinhart

Answer 12

Inorganic phosphate + glycerol

Question 13

A method that uses P-nitrophenyl phosphate as substrate

Answer 13

Bessy, Lowry, and Brock
Bowers and Mccomb

Question 14

Method that used phenyl phosphate as substrate

Answer 14

King and Armstrong

Question 15

Product of King and Armstrong Reaction

Answer 15

Phenol

Question 16

Reference range of ALP:

Adult

Answer 16

30-90 U/l

Question 17

Reference range of ALP:

0-3 months

Answer 17

70-220 U/l

Question 18

Reference range of ALP:

3-10 years

Answer 18

50-260 U/l

Question 19

Reference range of ALP:

10 yrs-puberty

Answer 19

60-295 U/l

Question 20

why is it ALP is increased in ages 3-puberty

Answer 20

Osteoblast Activity is Active

Question 21

What is used to differentiate ALP isoenzyme

Answer 21

Electrophoresis

Question 22

ALP Electrophoretic Migration:

Fastest to slowest

Answer 22

Love, BPI

Liver - Bone - Placenta - Intestine

Question 23

ALP isoenzyme:

Fastest to migrate towards anode

Answer 23

Liver ALP

Question 24

Liver ALP is consist of what factors

Answer 24

Major Type
Fast Liver

Question 25

Heat labile fraction of ALP isoenzyme

Answer 25

Bone ALP

Question 26

Bone ALP: temperature increases, ALP ____

Answer 26

Decreases

Question 27

Bone ALP is significantly increased in what conditions

Answer 27

Paget’s disease Ostitis deformans

Question 28

Clinical Significance of Bone ALP

Answer 28

Bone disease Healing of bone fracture Physiologic bone growth

Question 29

ALP isoenzyme: Heat stable fraction

Answer 29

Placental ALP

Question 30

Placental ALP is abundant at what stage of gestation

Answer 30

16th - 20th week of gestation

Question 31

Placental ALP is significant in what condition

Answer 31

carcinoplacental ALP

Question 32

Intestinal ALP is physiologically increased in what condition

Answer 32

After consumption of fatty meal (Blood type B and O)

Question 33

Intestinal ALP is pathologically increased in

Answer 33

GIT disorders

Question 34

Reagent that inhibits Placental and Intestinal ALP

Answer 34

Phenylalanine

Question 35

Reagent that inhibits Bone ALP

Answer 35

3 M urea

Question 36

Reagent that inhibits Bone and Liver ALP

Answer 36

Levamisole

Question 37

Test used to differentiate the elevation of Liver or Bone ALP

Answer 37

Heat stability Test

Question 38

Heat stabitility Characteristics of ALP

Answer 38

P>I>L>B

Question 39

Residual Activity decreased to >20%

Answer 39

Liver ALP

Question 40

Residual activity decreased to <20%

Answer 40

Bone ALP (heat labile)

Question 41

Heat stability test is done by

Answer 41

Heating serum @56 deg cel. For 10 mins

Question 42

Carcino Placental ALP are

Answer 42

Regan ALP Nagao ALP

Question 43

Most heat stable Placental ALP fraction

Answer 43

Regan ALP

Question 44

Regan ALP is found in

Answer 44

Lungs Breast Gynecological disorder Co-migrator of Bone

Question 45

Regan ALP can still act @what temp

Answer 45

65 deg cel. For 30 minutes

Question 46

Placental ALP fraction found in Adenocarcinoma of pancreas Bile duct Pleural Cancer

Answer 46

Nagao ALP

Question 47

Nagao ALP inhibitor

Answer 47

Phenylalanine and L-leucine

Question 48

Regan ALP inhibitor

Answer 48

Phenylalanine