Acid Phosphatase (ACP)

Question 1

Same with ALP (activity & function) differs in

Answer 1

pH and tissue source

Question 2

ACP function

Answer 2

hydrolysis of phosphomonoester at an ACID pH

Question 3

Production of alcohol is done through

Answer 3

liberating the inorganic phosphate from organic phosphate

Question 4

Major source of ACP

Answer 4

Prostate
Red Blood Cell
Platelet
Bones (osteoclast)
Seminal fluid

Question 5

Clinical significance of ACP

Answer 5

Prostatic cancer
Rape cases

Question 6

reason as to why ACP is not used as tumor marker for prostatic cancer

Answer 6

PSA (Prostate Specific Antigen) more specific

Question 7

Specimen use for Forensic investigation of rape

Answer 7

Vaginal Washing specimen

Question 8

ACP on seminal fluid only persist for up to

Answer 8

4 days (72 hrs)

Question 9

ACP Reaction

Answer 9

Phosphomonoester + H2O —> Alcohol + Phosphate ion

Question 10

use to differentiate specific ACP from nonspecific ACP

Answer 10

Inhibitors

Question 11

inhibits prostatic ACP

Answer 11

L-tartrate

Question 12

inhibits red blood cell ACP

Answer 12

Formaldehyde
Cupric ions

Question 13

Reference range: Prostatic ACP

Answer 13

0-3.5 ng/ml

Question 14

Enzymatic Assay for ACP

Answer 14

Bowers and Mccomb

Question 15

Reaction happens in Bowers and Mccomb reaction

Answer 15

p-nitrophenyl-phosphate —-> p-nitrophenol + phosphate ion

Question 16

p-nitrophenol end color

Answer 16

Yellow

Question 17

p-nitrophenyl-phosphate color

Answer 17

colorless

Question 18

Quantitative end point substrate

Answer 18

Thymolpthalein monophosphate

Question 19

Continuous monitoring substrate

Answer 19

a-naphthyl phosphate

Question 20

Substrate and End Product:

Gutman and Gutman

Answer 20

Phenyl PO4
Inorganic Phosphate

Question 21

Substrate and End Product:

Shinowara

Answer 21

PNPP (p-nitrophenyl phosphate)
p-nitrophenol

Question 22

Substrate and End Product:

Babson, Read and Philip

Answer 22

Alpha napththyl PO4
Alpha napthol

Question 23

Substrate and End Product:

Roy and Hillman

Answer 23

Thymolphtalein monophosphate
Free thymolpthalein