Lifescience

Question 1

What do you have to consider in different biosafety levels/contaiment levels?

Answer 1

• Lab/operational practices
• Safety equipment and PPE
• Lab design and construction

Question 2

What are the four hazard groups?

Answer 2

COSHH1 classifies the hazard groups as:
HG1: unlikely to cause human disease
HG2: can cause human disease and may be hazardous to employees
HG3: can cause severe human disease; it may spread to the community, but there is a treatment available
HG4: causes severe human disease, its likely to spread to community and there is no effective treatment available.

Question 3

What are some basic requirements of different containment level?

Answer 3

Cl1 - Labs must have a sink, surfaces must be wipeable, doors and access control.
CL2 - Self closing doors, BSC must be installed away from supply air, eye wash station should be available. Negative pressure recommended.
CL3 - Airlocks/lobbys required. anterooms, negative pressure, monolithic floor systems/sealed penetrations, cannot have recirculating exhaust air
CL4 - Clean and dirty corridors, completely sealed floors, walls and ceiling, HEPA filtration.

Question 4

What are some general lab requirements?

Answer 4

• Vulcathene drainage
• No floor voids

Question 5

What are the air change rates for different containment level?

Answer 5

Office 1-2
Cl2 - 5-6
Cl3 - 8+