LG 2.13 Neuronal Cell Death Flashcards
Describe the metabolic consequences of hypoxia/ischemia and the features of necrotic cell death?
Hypoxia-ischemia eliminated ATP production, leading to ionic imbalances that have vast metabolic consequences
Describe the morphological and molecular features of apoptosis?
Apoptosis causes laddering of DNA, karyolysis, and blebbing of cytoplasm.
Outline the contribution of apoptosis to ischemic brain injury?
Apoptosis can be triggered by cellular signals in injured cells, particularly by mitochondrial damage
Differentiate between necrosis and apoptosis/programmed cell death?
Oncosis leads to inflammation, apoptosis does not, but both can be triggered by hypoxia-ischemia
State the role of cell death in neural development and neurodegeneration?
Development of the neural system requires cellular rearrangement and pruning to create the complex cellular systems and morphology in the brain
How does apoptosis affect embryonic development?
- During embryonic development, the nervous system is “Sculpted” by neuronal cell death
- Excess neurons are removed to ensure proper and precise synaptic connections
Would regulation of apoptosis be considered the role of a proto-oncogene or a tumor suppressor gene?
Tumor suppressor gene
When does ischemia occur?
Ischemia of the brain usually occurs when blood flow drops below 25% of normal perfusion levels
What is another word for the core?
Umbra
What is the penumbra?
Outside the umbra
Treatment of a thrombo-emboli with a form of plasmin will save the neurons from damage as perfusion has been restored.
No. Most damage comes from reperfusion injury
What is excitotoxicity?
– nerve cells are killed by excessive neurotransmitters
What is oxidative stress?
reactive oxygen and nitrogen species destroy cellular structures
What is apoptosis?
programmed cell death
How do NMDA affect excitotociciy?
- NMDA receptors create fast excitotoxicity (>3 min) by increasing [Ca2+]i and interfering with calcium proteins in the cytoplasm
- Activation of calpains and cathepsins
- Activation of phospholipase A and C
- Activation of Calcium dependent protein kinases