lecture questions exam 3 Flashcards
which cell is the best to remove auto antibodies in patient plasma using adsorption procedures
- sc1
- sc2
- sc3
- patient cell
patient cell
-> reacts with self to reveal underlying alloantibodies
for elution procedures, which step ensures that the washing was appropriate
- addition of Coombs control cell after anit-AHG
- testing of last wash with screen cells
- testing of elute with panel cells
- color change from yellow to blue with buffer
testing of last wash with screen cells
what DAT results are most consistent with a diagnosis of CAD
positive poly reaction and C3d, negative IgG
- Poly is specific for compliment and IgG
- col autoimmune = IgM = IgG will be negative
penicillin given in massive doses has been associated with RBC hemolysis. Which of the classic mechanisms is typically involved in this hemolytic process
- Immune complex
- Drug adsorption
- membrane modification
- autoantibody formation
drug adsorption
which step of the elution procedure is different in comparing these three procedures: Lui-Freeze, Acid Elution and Heat Elution
process for antibody removal from the cell
- wash step in all 3 removes unbound antibody from plasma
which auto-antibody is indicated when DAT results are positive with anti-C3d but negative with anti-IgG
cold
___ detects antibodies on the cell in vivo, while ___ puts antibody on cells in vitro
- DAT: in vivo
- IAT: in vitro
true or false:
Quality control results for a neutralization procedure should produce negative agglutination reactions with panel cells
false
- neutralization quality control will show positive agglutination
- adsorption quality control will show negative agglutination
which of the following is NOT an interpretation from a negative agglutination reaction
- The Unite is compatible
- The Unit does not have the antigen
- The patient does not have the antigen
- the antibody is ruled in
the antibody is ruled in
which test would most help distinguish if the cold antibody identified is benign or pathologic
- test with cord cell
- test an enzyme-treated panel
- antibody titer
- DAT
antibody titer
based on listed properties, is this a cold or warm hemolytic anemia
- most common specificity is Anti-I
- DAT is C3 pos
- purple extremities
- intravascular hemolysis
- reacts at IS
cold
based on listed properties, is this a cold or warm hemolytic anemia
- extravascular hemolysis
- reacts at AHG
- DAT is IgG pos
- extremely low hemoglobin values
- most commonly anti-e
warm
if an antibody reacts at all phases but negative with cord cells it is most likely:
- anti-H
- anti-IH
- anti-i
- anti-I
anti-I
match the drug responsible for: drug adorsption
- cefotetan
- quinidine
- cephalosporin
- aldomet
cefotetan
- also streptomycin and penicillin
match the drug responsible for: immune complex
- cefotetan
- quinidine
- cephalosporin
- aldomet
quinidine
- also phenacetin
match the drug responsible for: membrane modification
- cefotetan
- quinidine
- cephalosporin
- aldomet
cephalosporin
match the drug responsible for: drug independent
- cefotetan
- quinidine
- cephalosporin
- aldomet
aldomet
if you have a DAT result that is IgG negative and compliment positive, and the serum in the sample is tea colored, what should you suspect is causing this
- WAIHA
- immune complex with a drug
- Darzalex
- nonspecific cold agglutinatin
immune complex with a drug
- drug induced antibodies appear like warm auto antibodies
- hemolysis is characterized by tea colored plasma
other than transfusion, what treatment might help a patient with autoimmunehemolytic anemia?
- plasma exchange
- antibody titer
- emergency release of O negative blood
- antibody adsorption
plasma exchange
- removes auto antibodies from patient
a positive donath landstener result indicates which diagnosis
- WAIHA
- PCH
- PNH
- CAD
PCH: paroxysmal cold hemoglobinruia
- anti-P in urethra
which of the following tests DOES NOT have to be performed for a patient to receive an electronic crossmathc
- Unit ABO retype
- blood type on previous sample
- antibody screen on current sample
- antibody screen on donor unit
anitbody screen on donor unit
- donor unit is only cells, cannot run an antibody screen
- abo retype on patient must be done and must match history
- patient must have a negative antibody screen
after a unit is issued, how long does the nurse have to infuse the whole product into the patient
- 30 min
- 2 hr
- 4 hr
- 24 hr
4 hours
- transfusion must take 4 or less hours
- transfusion can begin right before expiration date on donor bag and still be fine
which of the following components are acceptable to return to your blood bank inventory
- spiked, unused RBC w/ temp of 8C
- RBC w/ clot removed from the filter temp of 4.5C
- RBC with indicator dot red at 2.3C
- RBC out of blood bank for 30 min with white temp dot at 7.6C
rbc out of bank for 30min with white dot at 7.6C
which of the following samples are acceptable for add-on crossmatch?
- 4 days old, A pos, neg screen
- 1 day old, O pos, pos screen
- 2 days old, nu current screen results
- 3 days old, AB neg, neg screen, history of anti-Fya
1 day old, O pos, pos screen
3 day old, AB neg, history Fya
choose all that apply: under which circumstance would you warm a cross match
- A neg, neg screen , pos IS crossmatch
- O pos, anti-M ID
- O neg, anti-D ID
- ABO discrepancy, extra reverse reactivity
- A neg, neg screen, pos IS crossmatch
- O pos, anti-M
the last 3 patients in a row have given eng reactions with anti-A, this type of error is most likely:
- coincidence
- pt sample error
- systematic error
- delta error
systematic
