DAT Flashcards
when is a DAT ordered
- positive auto control
- hemolysis present
describe AHG polyspecific reagent
- anti IgG and anti - C3b
name the check cell with anti-IgG attached
coombs
name the check cell with c3 attached
compliment control cells
describe a positive DAT interpretation
- hemolysis from immune process
describe differential DAT
use of anti -IgG and separate anti-C3b
what is the next step if anti-C3b is positive
none, hemolysis due to compliment on patient’s red cells
what is the next step if DAT shows pos IgG
immune process placed Ab onto patient red cells
- elution
given following results, what is the type of IHA
- anti IgG pos
- anti C3b pos
WAIHA
HTR
given following results, what is the type of IH
- anti IgG pos
- anti C3b neg
WAIHA
HTR
HDN
given following results, what is the type of IH
- anti IgG neg
- anti C3b pos
CAS
PCH
when is elution preformed
pos IgG DAT with immune stimulating event
define the principle of elution
remove and identify antibody coating red cells
how is IgG removed from the cell
acid
freeze
heat
how is the integretiy of the elution checked
testing last wash against panel cells
-> should be neg, all antibodies removed