DAT Flashcards

1
Q

when is a DAT ordered

A
  • positive auto control
  • hemolysis present
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2
Q

describe AHG polyspecific reagent

A
  • anti IgG and anti - C3b
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3
Q

name the check cell with anti-IgG attached

A

coombs

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4
Q

name the check cell with c3 attached

A

compliment control cells

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5
Q

describe a positive DAT interpretation

A
  • hemolysis from immune process
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6
Q

describe differential DAT

A

use of anti -IgG and separate anti-C3b

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7
Q

what is the next step if anti-C3b is positive

A

none, hemolysis due to compliment on patient’s red cells

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8
Q

what is the next step if DAT shows pos IgG

A

immune process placed Ab onto patient red cells
- elution

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9
Q

given following results, what is the type of IHA
- anti IgG pos
- anti C3b pos

A

WAIHA
HTR

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10
Q

given following results, what is the type of IH
- anti IgG pos
- anti C3b neg

A

WAIHA
HTR
HDN

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11
Q

given following results, what is the type of IH
- anti IgG neg
- anti C3b pos

A

CAS
PCH

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12
Q

when is elution preformed

A

pos IgG DAT with immune stimulating event

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13
Q

define the principle of elution

A

remove and identify antibody coating red cells

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14
Q

how is IgG removed from the cell

A

acid
freeze
heat

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15
Q

how is the integretiy of the elution checked

A

testing last wash against panel cells
-> should be neg, all antibodies removed

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16
Q

when is auto adsorption run

A

panagglutination with no immune stimulating event
- no previous transfusion
- removes autoantibodies to reveal allo using red cells

17
Q

when is allo adsorption run

A

panagglutination with transfusion history
- removes allo antibodies to ID auto

18
Q

which test result differentiates between high incident antibody and auto antibody

A

auto control