DAT

Question 1

when is a DAT ordered

Answer 1

• positive auto control
• hemolysis present

Question 2

describe AHG polyspecific reagent

Answer 2

• anti IgG and anti - C3b

Question 3

name the check cell with anti-IgG attached

Answer 3

coombs

Question 4

name the check cell with c3 attached

Answer 4

compliment control cells

Question 5

describe a positive DAT interpretation

Answer 5

• hemolysis from immune process

Question 6

describe differential DAT

Answer 6

use of anti -IgG and separate anti-C3b

Question 7

what is the next step if anti-C3b is positive

Answer 7

none, hemolysis due to compliment on patient’s red cells

Question 8

what is the next step if DAT shows pos IgG

Answer 8

immune process placed Ab onto patient red cells
- elution

Question 9

given following results, what is the type of IHA
- anti IgG pos
- anti C3b pos

Answer 9

WAIHA
HTR

Question 10

given following results, what is the type of IH
- anti IgG pos
- anti C3b neg

Answer 10

WAIHA
HTR
HDN

Question 11

given following results, what is the type of IH
- anti IgG neg
- anti C3b pos

Answer 11

CAS
PCH

Question 12

when is elution preformed

Answer 12

pos IgG DAT with immune stimulating event

Question 13

define the principle of elution

Answer 13

remove and identify antibody coating red cells

Question 14

how is IgG removed from the cell

Answer 14

acid
freeze
heat

Question 15

how is the integretiy of the elution checked

Answer 15

testing last wash against panel cells
-> should be neg, all antibodies removed

Question 16

when is auto adsorption run

Answer 16

panagglutination with no immune stimulating event
- no previous transfusion
- removes autoantibodies to reveal allo using red cells

Question 17

when is allo adsorption run

Answer 17

panagglutination with transfusion history
- removes allo antibodies to ID auto

Question 18

which test result differentiates between high incident antibody and auto antibody

Answer 18

auto control