complex antibody ID leture

Question 1

define neutralization

Answer 1

uses a substance to bind the antibody to remove it from the system

Question 2

name the neutralizing substance for P1

Answer 2

Hydatid cyst fluid

Question 3

name the neutralizing substance of lewis

Answer 3

saliva

Question 4

name the neutralizing substance foe chido/rogers

Answer 4

normal human serum (compliment related)

Question 5

name the neutralizing substance for Sda

Answer 5

urine

Question 6

name the neutralizing substance for I

Answer 6

human breast milk

Question 7

define adsoprtion processes

Answer 7

uses red cell to bind an antibody to remove it from system

Question 8

name neutralization quality control and expected result

Answer 8

dilution control parallel test
- control agglutinates positive

Question 9

name adsorption quality control and expected result

Answer 9

• adsorbed serum against adsorbed cell
• control does not agglutinate

Question 10

describe mechanism of action for sulfhydryls (DTT)

Answer 10

• cleaves disulfide bonds
• destroyes kell
• breaks IgM off of cell

Question 11

describe mechanism of action of proteolytic enzymes

Answer 11

• removes sialic residues and denatures proteins
• removes duffy, MNS. JMH/ Ch/Rg and Xga

Question 12

describe mechanism of action of ZZAP

Answer 12

• combination of DTT and enzyme
• cystein-activated papain
• acts as both DTT and enzyme
  -> cleaves disulfide bonds and removes sialic residues

Question 13

describe mechanism of action for quinolines

Answer 13

• splits immune complexes by reducing van der walls

Question 14

describe mechanism of action for glycine-acid EDTA EGA

Answer 14

• dissociates RBC bound IgG via pH for select RBC antigen destruction
  -> Kell, Cartwright, Dombrock and knops

Question 15

list false pos of phenotyping

Answer 15

• transfused cells
• positive DAT

Question 16

list false neg of phenotyping

Answer 16

• partial antigen expression
• heterozygous expression
• anti-sera