Lecture B3, B4 Flashcards
Digestive system parts: _____
The digestive tract: Oral cavity, esophagus, stomach, small and large intestine, anus
Associated Glands: salivary glands, liver, pancreas
digestive system comes from which germ layer?
endoderm
endoderm forms ______
foregut, midgut (small intestine), hindgut (colon)
endodermal cells form a _____ which molds into an epithelial tube surrounded by _____. This process requires many different signaling pathways and multiple transcription factor genes encoding proteins involved in ____
-polarized sheet
-mesoderm
-adhesion, the cell cycle and differentiation
Signaling pathways that regulate differentiation of the
different areas of the digestive tract
-Wnt/FGF4,BMP from mesoderm (increases down the digestive tube)
Four main layers of small intestine
- Mucosa (mucous membrane): Epithelial lining, Lamina propria – loose connective tissue, Rich in blood vessels, Thin smooth muscle layer (muscularis mucosae)
- Submucosa, connective tissue, links to underlying muscle layers
- Muscularis: inner layer (circular), outer layer (longitudinal)
- Serosa: outermost layer connective tissue, blood vessels, adipose
Mesentery surrounds the _____ and suspends them from _____
-stomach, small and large intestine
-the abdominal wall
Movement of nutrients from the lumen of small intestine to be absorbed into the bloodstream happens across the _____. Transcellular transport requires: (2)
-epithelial cells
-Membrane transport proteins in the apical surface (active
transport) & the basolateral surface (facilitated diffusion)
the small intestine folds ____ to create _____
-mucosa and submucosa (epithelial lining)
-dense apical fingerlike projections called villi to increase absorptive area
villi are covered by _____ and contain ____
-columnar epithelial cells
-goblet cells and absorptive enterocytes
each villus is connected to _____ which contains ____
-the lamina propria
-the capillary network
enterocyte/absorptive cell has ____ to increase absorption
-dense microvilli (brush border) on apical surface
circular and longitudinal muscle layers of small intestine are responsible for ____
peristalsis
SC of intestine is marked by ____ and differentiates into ___ then into _____
-LGR5+
-TA cells
-enterocytes, goblet cell, tuft cell, enteroendocrine cell, paneth cell
SC of intestine & paneth cells are in _____ and move up the villus when differentiating
-crypts
BMP signalling increases _____
up the villus
WNT signalling increases _____
down the villus (in the crypt)
WNT signalling in villi does what? Notch? BMP? Hh?
-maintains ISCs, Paneth cells
-differentiation of enterocytes
-differentiation of enteroendocrine cells, keeps stem cell from dividing too much
-Sense epithelial integrity
Niche of ISC
-ECM
-Signaling molecules
-Basement membrane
-Physical factors
-Paneth cells support intestinal stem cells (ISCs) via secretion of Wnt, Notch (DLL) and EGF
After injury to intestine, Progenitors and also enterocytes can dedifferentiate into ______
-LGR5+ intestinal stem cells (ISCs) through WNT-mediated niche signals
absorptive intestinal surface forms from ____ signalling (2) + function
-Wnt5 (Highly proliferative during tube elongation -> cells grow in height just prior to villi formation)
-Hh (Villi emergence)
after Hh signalling, cells at tip of villus transition have ______ while cells are inter-villi region remain _____
-simple columnar structure (expanding apical surface)
-pseudostratified
selective absorption of intestine requires _____, giving the gut structure to resist sheer forces in the tube
TJ, AJ, Desmosomes, Hemidesmosomes
what we know about microvilli (3)
-They are dynamic – always recycling actin filaments.
-their structure and the role of actin and actin binding proteins.
-Ezrin plays an important role.
defects in villi formation or structure causes this disease?
Celiac disease
Microvilli are made of _____
- bundles of actin filaments
-Actin binding accessory proteins like Myosin delivers components
-Actin filaments insert into Terminal web or intermediate
filaments
-Formin and other proteins cap F-actin at the top
Microvilli formation during enterocyte differentiation
-Microvilli initially appear as small membrane buds on apical surface
-Protrusions self organize into distinct clusters mediated by adhesion between distal tips
-Distal Tip adhesion promotes packing of bundles together to form an organized structure
-actin filament cores attach to actin binding bundling proteins (fimbrin, villin, espin) and are cross linked to apical membrane by Ezrin
enterocytes absorb _____
lipids, proteins, sugar components
lipid absorption by enterocytes
-Hydrolyzed lipid components enter the cell by passive diffusion
-lipids accumulate in SER then fatty acids are resynthesized as triglycerides
-TGs are processed through the RER and
Golgi and covered in a thin protein layer to form chylomicrons (larger lipid droplet) near the nucleus
-These are exocytosed from the lateral membrane
sugar absorption by enterocytes
-Secrete disaccharidases into the glycocalyx (ECM)
-Hydrolyses disaccharides into monosaccharides
-monosaccharides are actively transported across microvilli and deposited in the underlying capillaries (sodium dependent)
protein absorption by enterocytes
-Secrete peptidases into the glycocalyx (ECM)
-Hydrolyses dipeptides into monopeptides
-monopeptides are actively transported across microvilli and deposited in the underlying capillaries
Goblet cells are essentially _____
a single cell gland (merocrine)