Lecture 8 Questions Flashcards
What happens to proteins after their ribosomal synthesis ?
Folding
PTMs
Transport
Complex assembly
Which types of bonds stabilize which types of structure ?
Primary structures: peptide bonds
secondary structure: Hydrogen bonds
Tertiary bonds: Hydrogen bonds
Van der Waals focres
Hydrostatic interaction
What is the energetics of protein folding ?
Entropy: folding to be in the most advantageous state possible
What kind of tasks are taken over by chaperone proteins ?
Prevent aggregation
Assists in folding
Transportation
What happens to a protein that becomes unfolded?
Refolded and if it cant be refolded it is degraded
What are the biological reasons for proteins requiring maturation
Post-Translational Modifications (PTMs)
Folding and Assembly
Localization
Complex formation
maintaining cellular homeostasis and responding to environmental cues.
What determines the localization of a protein?
Signal Sequences (Poly A tail)
PTMs (ubiquitination)
KDEL signal
What are the folding/unfolding requirements for organellar protein import?
Target signal
Unfolding for import into Mitochondria
Refolding/folding after translocation
How are local minima overcome in folding?
By chaperones
