Lecture 8 - Pyridoxal Phosphate Mechanism

Question 1

What is the first step of the PLP mechanism?

Answer 1

Amine group of amino acid attacks the aldehyde of PLP

Question 2

What is the second step of the PLP mechanism?

Answer 2

Loss of water and rearrangement of double bonds

Question 3

What is the third step of the PLP mechanism?

Answer 3

Addition of water across the C=N bond leaving an OH on the C

Question 4

What is the fourth step of the PLP mechanism?

Answer 4

OH on the C forms C=O and dissociates itself from the N, releasing pyruvate

Question 5

What is the fifth step of the PLP mechanism?

Answer 5

NH2 on the PLP goes through the reverse reaction, attacking the ketone group of alpha-ketoglutarate

Question 6

What is the sixth step of the PLP mechanism?

Answer 6

Water is released producing a C=N bond

Question 7

What is the seventh step of the PLP mechanism?

Answer 7

Rearrangement of electrons, followed by addition of water across the double bond

Question 8

What is the eighth step of the PLP mechanism?

Answer 8

Formation of an aldehyde group on PLP and release of glutamate

Question 9

What is the cofactor of step 1?

Answer 9

H+ out