Lecture 8

Question 1

Describe some features of K. pneumonia.

Answer 1

rod-shaped, non-flagellated, G-, facultative anaerobe, encapsulated, and is non-spore forming

Question 2

What are the four major virulence factors of K. pneumonia?

Answer 2

capsule, lipopolysaccharide, fimbriae, and siderophores

Question 3

What is proteomics?

Answer 3

the entire protein complement expressed by a genome

Question 4

What is mass spectrometry-based proteomics?

Answer 4

used to identify proteins

Question 5

What are the two methods of mass spectrometry based proteomics?

Answer 5

bottom-up proteomics - proteins are digested into peptides prior to analysis
top-down proteomics - looks at whole proteins

Question 6

What is the protein preparation workflow?

Answer 6

extraction, digestion, purification, identification, and data analysis

Question 7

What is nutritional immunity?

Answer 7

the host will sequester any free metal to restrict bacterial growth