Lecture 7 Flashcards
Who was the first to find the source of a disease?
John snow, he used compared a map of diseased individuals to a map of water pumps
Describe Vibrio cholera statistics.
G-, facultative anaerobe, forms yellow colonies, has one flagella, and has short generation time
What are cholera symptoms?
few hours after infection cramping and diarrhea start, lots of fluid loss
How is cholera treated?
through monitoring and hydration
What is vibrio cholera’s main virulence factor?
cholera toxin (CTX)
Describe the cholera toxin.
AB5 toxin, ADP-ribosylating toxin, lysogenic bacteriophage
What is the cholera toxin mode of action?
5B;1A binds to host membrane
toxin is endocytosed
the CTX is taken to the endoplasmic reticulum
the A1 subunit is removed and exported to the cytoplasm
the A1 attached an ADP ribose to an AA
cAMP levels rise and activates ion transport systems causing water to leave cell (diarrieha)
Describe the features of e.coli.
- G-
- facultative anaerobe
- has flagella
What is a serotype?
an antigenically distinct group
What is a pathotype?
a term used to describe a group of strains of species that cause a common disease using a common set of virulence factor
What does the O serotype mean?
has LPS
What does the H serotype mean?
has flagella
What does the K serotype mean?
has capsule
What are the virulence determinants of e.coli?
pedestal formation and production of shiga toxin
What is the shiga toxin?
an AB5 toxin that inhibits protein synthesis in host cell
