Lecture 7 Flashcards
2 prokaryote domains
- Archaea
2. Bacteria (distinguished by RNA structure)
7 characteristics of prokaryotes
- evolved before evolution of nucleus
- plasmids
- reproduction and gene transfer takes several forms, none using mitosis or meiosis
- cell wall (when present) includes peptidoglycans
- lack membrane-enclosed organelles
- smaller than eukaryotic cells
- half are motile (using flagella)
nucleus
genetic material as dense linear chromosomes enclosed by membrane, present in eukaryotes
nucleoid region
diffuse, circular ring of DNA not enclosed by membrane, present in prokaryotes
plasmids
small rings of DNA containing a few ‘extra’ genes
4 types of reproduction and gene transfer in prokaryotes
- binary fission
- transformation
- transduction
- conjugation
binary fission
most common, chromosome duplicates and cell splits
transformation
uptake of ‘naked’ DNA released from dead bacteria by a living prokaryote
transduction
virus mistake - transportof DNA between prokaryote cells by viruses
bacteriophage
virus that infects and replicates with bacteria
conjugation
one prokaryote latches on to another with a string-like structure (pilus) and transfers DNA (unidirectional)
why can antibiotic resistance spread so quickly?
modes of horizontal gene transfer
horizontal gene transfer
between individuals of the same generation - transformation, transduction, conjugation
vertical gene transfer
transfer from parent to offspring between generations - binary fission
peptidoglycans
not cellulose or chitin, prevents cell from exploding in hypotonic solution
plasmolysis
contents of cell shrink in hypertonic solution, not prevented by cell wall
Gram + bacteria
have peptidoglycan wall in contact with external medium which traps purple stain
Gram - bacteria
have lipopolysaccharide layer outside of cell wall, and thus do not absorb stain readily (more pathogenic)
taxis
movement toward or away from stimulus, demonstrated in many prokaryotes
metabolic pathways
differ in prokaryotes and eukaryotes
metabolism
chemical pathways used by living organisms to build up
or break down molecules