Lecture 6 - Asepsis Flashcards
What is asepsis?
Absence of microorganisms that cause disease
What is aseptic technique?
Method to prevent contamination by microorganisms;
“clean” technique
What does it mean if something is sterile?
It is free of all living organisms
What is sterile technique?
Method by which contamination with microorganisms is prevented;
performed in a sterile field
What is a pathogen?
Microorganism that causes infection; can be human or animal
What is infection?
Multiplication of an agent within the tissues that results in cellular injury
What is nosocomial infection?
Infection resulting from hospital treatment;
AKA hospital-acquired infection (HAI)
What is surgical asepsis?
Procedures used to keep the object or area sterile or free of microorganisms.
What are all practices directed to eliminate?
Pathogenic and non-pathogenic microorganisms
What is the most common type of nosocomial infection?
Surgical site infection (SSI)
Surgical site contamination with _____ microorganisms per g of tissue increases risk of SSI.
> 10^5
What is often the source of SSIs?
endogenous flora
How can SSIs be diagnosed?
Will have purulent discharge, inflammation, can isolate microorganism
What are common bacteria that cause SSIs?
Staphylococcus aureus, Streptococcus, Enterobacter, Enterococcus, E. coli
Staph aureus can be found in nares of _____ healthy humans.
20-30%
What are the 3 types of SSIs?
- Superficial incisional SSI
- Deep incisional SSI
- Organ/Space SSI
During what time period can superficional incisional SSIs develop?
Within 30 days of sx
What tissues do superficial incisional SSIs affect?
Skin/SQ
What signs are seen with superficial incisional SSIs?
Purulent drainage, culture, inflammation
During what time period can deep incisional SSIs develop?
Within 30 days of sx; within 1 year with implant
What tissues do deep incisional SSIs affect?
Deep tissue (fascia/muscle)
What signs are seen with deep incisional SSIs?
Purulent drainage, abscess, fever/pain
During what time period can organ/space SSIs develop?
Within 30 days of sx; within 1 year with implant
What tissues do organ/space SSIs affect?
organ or space
What signs are seen with organ/space SSIs?
Purulent drainage, abscess, fever/pain
SSIs increase _____ and _____ rates.
morbidity, mortality
SSIs occur in _____% of humans
10-15
SSIs occur in _____% of small animals.
1-18
SSIs occur in _____% of equines.
1-50
What are prophylactic antimicrobials?
Brief course of antimicrobials administered immediately before a surgical procedure.