LECTURE 6 Flashcards
Peptidoglycan
A thin sheet composed of
1) chains of repeating disaccharide unit composed of two monosaccharides
2) small peptides
What are the two monosaccharides that compose the repeating chain of disaccharides in peptidoglycan
1) N-acetylglucosmine (NAG)
2) N-acetylmuramic acid (NAM)
Small peptides that make up peptidoglycan
- attaches to NAM peptides in adjacent chains, cross-linking the chain, making them stronger
Where does the short peptide get added to?
Gets added to the NAM
How do tw peptide side chains attach to each other
Via covalent bonds
Transpeptidation
The cross linking reaction of two peptide chains, this adds strength
Is peptidoglycan a barrier to solutes, why or why not?
No, because the opening are to large.
As. A bacteria grows, peptidoglycan
Is produced more.
Some antibiotics are specific to certain bacteria, what is this based on.
Based on their ability to target prokaryotic-specie structures.
Two common targets for antibiotics are
1) Prokaryotic ribosomes
2) Prokaryotic cell walls
What does it mean for a cell to lyse ?
For it to burst
Lysozyme
An enzyme that catalysts hydrolysis of B 1-4 linkages between NAG and NAM
Peptidoglycan falls apart, causing the cell to lyse.
Staphylococcus
Gram positive bacterium
As bacterial cells grow, it synthesizes more
Peptidoglycan
How does penicillin work?
1) transpeptidation is catalyze by a specific enzyme
2) penicillin inhibits this enzyme
3) peptidoglycan weakens
4) eventually the cells bursts (lysis)
What does penicillin work best on ?
Gram positive cells
Many bacteria besides having a cell wall also have a
Capsule
What is a capsule made of
Mostly polysaccharides
Capsules functions
1) protects from environment
2) stick bacteria to surface
What i sa capsule rare is
Archaea
Two types of pili
1) Fimbriae
2) sex pilus
Fibriae
Attaches to surface of host cells
Gonorrhoea uses fumbriae to attached itself to mucus membranes
Sex pilus
Transfers DNA or plasmid between bacteria cells
Bacterial conjugation
DNA is transfers to another bacteria