LECTURE 16 Flashcards

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1
Q

Cytokinesis

A

The plotting of cytoplasm in a eukaryote

  • begins in late anaphase and continues through telophase
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2
Q

What’s forms at the membrane during cytokinesis

A

An actin-based contractile ring

  • myosin motors use ATP to pull on the actin filaments to separate the cytoplasm.
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3
Q

What is the position of the cleavage furrow determined by

A

Mitotic spindle

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4
Q

In plant cells, nuclei seperate in mitosis, and then a ________forms during cytokinesis

A

Cell plate

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5
Q

The cell plate develops into the

A

Cell wall that divides the two cells

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6
Q

Cell cycle control

A

Regulating cell division in eukaryotes

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7
Q

Which checkpoints in interphase are the most important

A

G1

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8
Q

What are the 3 major checkpoints in interphase

A

1) G1/S checkpoint
2) G2/M checkpoint
3) mitotic (spindle assembly) checkpoint

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9
Q

G1/S checkpoint

A
  • is there a single to divide?

- checks for a growth factor and if there isn’t one it enters G0 phase

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10
Q

G0 phase

A

A non dividing phase

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11
Q

External singles

A

Signal to divide

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12
Q

Internal signals

A

Large enough or enough nutrients

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13
Q

G2/M checkpoint

A
  • DNA replication complete?

- DNA free of errors?

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14
Q

Mitotic (spindle assembly) checkpoint

A

Are all kinetochore s connected to the ends of microtubules; problems may occur.

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15
Q

What type of problems may occur if kinetochores dont attach properly to microtubules

A

A) both kinetochore aren’t attached

B) one kinetochore isn’t attached

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16
Q

What do impoper conditions in kitechore attachment prevent?

A

They prevent entry to anaphase; the cell will keep trying to make proper connections until both are connected.

17
Q

Specific proteins at the kinetochore sense if a microtubule is attached; if it is not, then a. Signal inactivated the

A

APC, this prevents destruction securin and prevents anaphase

18
Q

Why do cell device in serum and not plasma ?

A

It wasn’t due to the nutrients in serum, but something that had to do with blood clotting processes.

19
Q

Fibroblasts make

A

collagen and other ECM components like fibronectin. When a wound occurs, fibroblasts divide and secrete ECM components to the wound

20
Q

What is the protein that promotes cell division called

A

Platelet-derived growth factor

blood clotting requires platelets

21
Q

Mutations occur ________ at _____ rates

A

Spontaneously Slow

22
Q

Was the transformation permanent and/or heritable in Griffiths experiment

A

YES

23
Q

Transformation

A

Change in genotypes and phenotype due to assimilation of foreign DNA

24
Q

Viruses that infect bacteria are called

A

Bacteriophages

25
Q

Bacteriophages

A
  • cannot replicate on their own
  • can transform bacteria
  • made of nuclei acid (DNA) and proteins.
26
Q

What to use to track the movement of DNA and protien after the virus infects the bacteria ?

A

Radioactive isotope

27
Q

Which isotope to use to trach movement of DNA and why?

A

Use P32 because its incorporated in DNA which is what is transferred