Lecture 5-7 Microscopy Flashcards
Number of nanometers in a micrometer
1000
Average size of bacteria
1-2 micrometers (1000-2000 nanometers)
Unit of size for viruses
nanometers
Number of micrometers in a milimeter
1000
Number of lenses a simple microscope has
1
What a light microscope uses to observe things
light
Number of lenses a compound microscope uses
2
Type of light microscope most commonly used in labs
brightfield microscopy
Purpose of ocular lens
the eyepiece; remagnifies image (10x, 20x)
Purpose of objective lens
primary lens that magnifies the specimen (1x - 100x)
Purpose of the stage
holds microscope slide in position
Purpose of condesner
focuses light through specimen
Purpose of diaphragm
controls the amount of light entering condenser
Purpose of illuminator
the light source
Total magnification =
objective lens x ocular lens
Resolution
ability to distinguish between two adjacent objects; very very important
Resolution of compound light microscope
0.2 micrometers
Shorter wavelengths of light =
greater resolution
Refraction
bending of light through different substances
Need immersion oil when
using a magnification above 100x
Purpose of immersion oild
helps capture beam of light and prevents it from bending off