Lecture 30 - Fever and Lymhadenopathy

Question 1

Causes of lymphadenopathy?

Answer 1

response to locl infection, malignant cells that have metastasised to lymph node, proliferation of malignant lymph node, inflammation within node from killing of virus infected cells

Question 2

Bacterial infections of lymph nodes?q

Answer 2

s. aureus, m. tuberculosis - regional

Question 3

Viral infections of lymph nodes?

Answer 3

EBV (epstein barr virus), CMV (cytomegalovirus), HIV - diffuse

Question 4

Acute EBV infection?

Answer 4

glandular fever - minor as child severe as adolescent+, saliva transmission, incudbation 4-6wk, 1-2wk illness, fever, sore throat, cervical lymphadenopathy, malaise, fatigue

Question 5

Laboratory diagnosis of Acute EBV infection?

Answer 5

lymphocytosis, atypical lymphocytes, abnormal liver function tests, monospot test (heterophile antibodies), specific EBV serology

Question 6

Laboratory diagnosis of Acute CMV Infection?

Answer 6

lymphocytosis, atypical lymphocytes , abnormal liver function tests, detection of antibodies to CMV, CMV detection in blood

Question 7

Acute HIV infection?

Answer 7

recent (3-6 weeks) exposure, glandular fever-like symptoms, persisten viraemia and virus in genital secretions, presence of antibod to HIV in blood

Question 8

HIV reverse transcription?

Answer 8

watch the films mother fucker

Question 9

Time course of untreated HIV infection?

Answer 9

see book

Question 10

Enzyme Linked Immunosorbent Assay (ELISA) process?

Answer 10

HIV antigen stuck to base of ELISA wells, serum sample added (antibody will stick if positive), anti-human antibody w adhesive added (attaches to HIV antibody if +ve), reagent added (if positive, cleaved by stuck anti-human antibody resulting in bright blue)

Question 11

ELISA weak positive?

Answer 11

may have only just started forming antibodies, retest i wks, reversal means cross-over of different antibody (false +ve)