Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BPM DLA1 > Lecture 15 > Flashcards

Lecture 15 Flashcards

1
Q

First step of translation (Activation of monomer)

A

Amino acid is adenylated with AMP moiety from ATP. Then, adenylated amino acid is attached to tRNA by amino acyl-tRNA synthase with high bond to form aminoacyl-tRNA (also called charged tRNA).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What does initiation of translation need?

A

Initiation requires initiator tRNA that has fMET in prokaryotes and Met(methionine) in eukaryotes, and AUG start codon.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Describe preparationg of starting tRNA in initiation of translation in prokaryotes

A
  1. Met is attached to initiator tRNA.

2. Met is then formylated by transformylase to form fMET-tRNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is special about fMet-tRNA

A

Methionine attached to tRNA that is not the initiator tRNA cannot be formylated.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the formyl donor in initiation of translation?

A

N10-formyl-H4folate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How does 16S subunit of rRNA bind to mRNA for initiation?

A

There shine-dalgarno sequence (purine rich mRNA) that occurs 6-10 bases before AUG. 16S subunit contains complementary nt sequences to Shine Dalgarno seq which directs the binding and positioning of mRNA on ribosome.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Describe formation of initiation complex in prokaryotes (4)

A
  1. Small subunit binds to mRNA using Shine-Dalgarno seq on mRNA
  2. initiation factors attach to mRNA.
  3. Charged initiator tRNA loads onto AUG in the P site by IF2-GTP
  4. 50S subunit binds to form 70S complex. Energy required in this process is generated by hydrolysis of GTP and result in release of Initiation factors.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Describe elongation of translation in prokaryotes (5)

A
  1. Next aminoacyl tRNA is delivered to the empty A site by EF-Tu-GTP (Elongation factor)
  2. Then, GTP is hydrolyzed and EF released
  3. Then, energy from breakage of bond between carboxyl group of amino acid and the tRNA (tRNA charging) in P site form a peptide bond between 2 amino acids on A site, catalyzed by ribozyme (enzymatic activity of RNA portion of large subunit)
  4. EF-G-GTP translocates the ribosome 3 nucleotides along the mRNA. As a result deacylated tRNA (no aa) is shifted to E site and released while dipeptide-tRNA is shifted into P site.
  5. Steps are repeated until a termination codon is encountered in A site.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the name of EF-Tu-GTP in eukaryotes?

A

EF-1A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the name of EF-F-GTP in eukaryotes?

A

EF-2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is Diptheria and what is the cause of the disease

A

It is acute infectious disease affecting the upper respiratory tract and occasionally the skin, caused by toxin A from C ulcerans.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Where is Toxin A from and what is its effect

A

Toxin A is produced by lysogenic bacteriophage (beta phage) which infects bacteria. It catalyzes the transfer of ADP-ribose to host cells EF-2 (same function as EF-F-GTP), inactivating it and preventing translocation of ribosome, thus inhibiting protein synthesis.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the treatment of toxin A?

A

Nicotinamide to reverse reaction + antibiotics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Which stop codon does Release factor 1 recognize in termination of translation?

A

UAA, UAG

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Which stop codon does Release factor 2 recognize in termination of translation?

A

UAA, UGA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Role of release factor 3 in termination of translation

A

Bound to GTP, it carries out GTPase activity.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What happens in termination of translation

A

When stop codon is in A site, release factor enters the A site, triggering the peptidyl transferase to hydrolyse the ester bond between tRNA and completed polypeptide chain. Then, tRNA and ribosome subunits are recycled.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Role of polysome (polyribosome)

A

increase efficiency of protein synthesis as more than 1 ribosome can translate 1 mRNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

In prokaryotes, transcription and translation are coupled?

A

Yes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What protein carries out protein folding during translation?

A

Chaperones

21
Q

Mechanism of streptomycine

A

inhibit initiation of translation by binding to 30S subunit, distorting its structure, which prevent binding of fMET-tRNA to the P-site of ribosome. Thus, this inhibits initiation thus assembly of full ribosome.

22
Q

What bacteria does streptomycine target?

A

gram ‘-‘ such as TB

23
Q

Mechanism of tetracycline

A

Inhibits elongation by interacting with 30S and 40S small ribosome subunits blocking access& therefore binding of aminoacyl-tRNA to A site.

24
Q

Advantage of tetracycline

A

Specific for prokaryotes because eukaryotic cells doesn’t let tetracycline diffuse into the cell.

25
Q

Mechanism of Erythromycin

A

Inhibit elongation by binding irreversible to 50S ribosome (large subunit), blocking translocation by EF-G, thus preventing elongation.

26
Q

Which bacteria does erythromycine target?

A

gram ‘+’

27
Q

Disadvantage of erythromycine

A

Resistance can occur through plasmid controlled alteration of the binding site for erythromycine on the ribosome.

28
Q

Mechanism of chloramphenicol

A

inhibit elongation by binding to 50S ribosome and inhibiting peptidyl transferase activity, preventing peptide bond formation, thus preventing elongation.

29
Q

Disadvantage of chloramphenicol

A

it can cause serious haematological toxicity since it may inhibit eukaryotic mitochondrial ribosome, so it is not used in developed countries

30
Q

Mechanism of cycloheximide

A

inhibit elongation by inhibiting 60S subunit in eukaryotes and inhibiting peptidyl transferase activity, thereby preventing peptide bond formation , preventing elongation.
*** 60S = Eukaryote so this is used only for research

31
Q

Why is cycloheximide used for research?

A

Translation by mt ribosome is unaffected by cycloheximide, so to study mt translation, cycloheximide is used.

32
Q

Mechanism of puromycin

A

Puromycine is analogue of phenylalanine attached to the tRNA. It has amide bond instead of ester linkage, thus once incorporated, ribosome (Peptidyl transferase) cannot break amide bond to release this tRNA, inhibiting elongation and causing premature chain termination.

33
Q

Disadvantage of puromycin

A

active in both eukaryote and prokaryotes

34
Q

Types of post-translational modification (6)

A
  1. Folding or formation of disulfide bridge
  2. Covalent alteration (phosphorylation, glycosylation, hydroxylation)
  3. Proteolytic processing such as trimming
  4. protein degradation (ubiquitination)
  5. Addition of prosthetic group (e.g. heme)
  6. Prenylation (eg. lipid anchoring, farnesylation)
35
Q

Describe phophorylation as post translational modification

A

Transfer of phosphate group from ATP to amino acid side chain by kinase
- usually occurs on OH group of serine or threonine or less frequently tyrosine residue

e.g. insulin tyrosine kinase receptor

36
Q

2 types of glycosylation

A
  1. O-linked glycosylation

2. N-linked glycosylation

37
Q

What is O-linked glycosylation

A

Carbohydrate chains are attached to serine & threonine OH group. Usually occur after protein reach the golgi apparatus.

38
Q

What is N-linked glycosylation

A

Carbohydrate chains attached to amide nitrogen of asparagine. Usually occurs in ER& Golgi. Catalyzed by glycosyltransferase

39
Q

What is role of glycosylation?

A

Alters the properties of proteins, changing their stability, solubility and physical bulk. Also it act as a recognition signals that direct protein targeting to either plasma membrane or to organelle interiors or organelle membrane.
Also as a recognition signal, it influence cell to cell interaction.

40
Q

Lipid anchoring as post-translational modification

A

Example : RAS protein
Farnesyl (15 carbon isoprenoid group) is attached to the cysteine of protein by farnesyltransferase.
Then, RAS is anchored to cytosolic face of cell membrane through farnesyl group.

41
Q

What is proteolytic processing used for as post-translational modification?

A

zymogen activation to prevent premature activation of hydrolytic enzymes

42
Q

Example of zymogen activation

A

Trpsinogen is cleaved into trypsin by enteropeptidase in small intestine (enteropeptidase expression happen only in small intesting to prevent autocatalytic pancreatitis).

43
Q

Example of insulin as zymogen activation

A

Preproinsulin contains amino-terminal signal sequence that targets polypeptide chain to ER. Then, removal of signal sequence yields a second precursor, called proinsulin that has C-peptide. Then proinsulin is converted to insulin by proteolytic removal of C-peptide at Golgi body.

44
Q

Ribosome configuration for prokaryotes and eukaryotes

A

Prokaryote : 50S+30S = 70S

Eukaryote : 60S + 40S = 80S

45
Q

Difference in initiator tRNA between prokaryotes and eukaryotes

A

prokaryote : carry fMet

eukaryote : carry Met

46
Q

Difference in setting correct reading frame in the beginning of translation between prokaryotes and eukaryotes

A

Prokaryote : Shine dalgarno seq

Eukaryote : scanning mechanism

47
Q

Initiation factor of prokaryotes and eukaryotes

A

prokaryote : IF 1,2,3

Eukaryote : IF2 + additional factor

48
Q

Elongation-aminoacyl tRNA delivery of prokaryotes and eukaryotes

A

prokaryote : EF-Tu-GTP

Eukaryote : EF-1-GTP

49
Q

Elongation - translation factor of prokaryotes and eukaryotes

A

Prokaryote : EF-G-GTP

Eukaryote EF-2-GTP

BPM DLA1 (47 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions