Lecture 14 Flashcards
Where are genes found?
chromosomes
Genetic information is located on…
DNA
DNA replicates into which fashion?
semi-conservative
What did Frederick Griffith discover?
Traits can be transferred between different bacterial strains
What is the experiment of Frederick Griffith?
he injected a group of mice with live S strain (pathogenic bacteria), the mice died.
he injected of living R (nonpathogenic)… mice survived
he injected killed S (nonpathogenic)…mice survived
he injected living R strain with dead S strain… mice died
What do bacteria exchange?
plasmids
How much monomers do proteins have? How about DNA?
20 and 4
What was the experiment of Avery, MacLoed and McCarthy?
They purified cell extracts (proteins, DNA, RNA, carbohydrates, lipids) from dead S strain bacteria. They incubated the extracts with R strain cells. The strain that carried the DNA transferred the S characteristic.
What was showed with the experiment of Avery, Macloed and McCathy?
DNA is the transforming factor
What atoms is found in proteins but it is not found in DNA?
sulphur
What atoms is found on the DNA but not in proteins?
phosphorous
In a virus where is DNA found?
inside the head, the rest is proteins
What is the experiment of Hershey and Chase?
They put two different bacteriophages, one with radioactive S36 and one with P32. You inject bacteria with each bacteriophage, then they are blended to remove the viruses. This is centrifuge and it gives a supernatant (virus proteins) and pellet (bacterial cell contents) is heavier than protein.
What is the experiment of Chargaff?
He compared the DNA of various species using paper chromatography.
What did it showed?
A-T,G-C
