Lecture 10.27.14 (Exam #3) Flashcards
If there is oxygen present then pyruvate is sent to_____
citric acid cycle
The outer membrane of the mitochondria is_____
very purous
The inner membrane of the mitochondria is_____
tightly sealed and needs transporters to get molecule across
Inner membrane space
space between the inner and outer membrane
Matrix
space inside the inner membrane
What enzyme moves PA from the inner membrane space to the matrix?
pyruvate translocase
What also happens when PA is moved into the matrix?
H+ is moved into the inner membrane space
Antiport
One molecule in while another molecule is sent out
ex.PA and OH-
PA is converted to Acetyl CoA and CO2 by which enzyme?
Pyruvate decarboxylase complex
CoA
a mobile carrier of carbons
enzyme complex
group of enzymes, proteins, cofactors that are all coupled together
How many enzymes are apart of PDH?
5
What are the jobs of the 5 enzymes of PDH?
3 used for cataylsis
2 regulate later
3 catalytic enzymes of PDH?
pyruvate dehydrogenase (E1) acetyl transferase (E2) dehydrogenase (E3)
Pyruvate dehydrogenase does what and what is its PG?
redox
TPP
Acetyl transferase does what and what is its PG?
transfer functional group
lipoamide
Dehydrogenase (E3) does what and what is its PG?
redox
FAD
Pyruvate Dehydrogenase binds ____ to ___ and CO2 is lost.
acetate group to TPP
decarboxylation and alpha cleavage
Acetyl transferase (E2)’s ______ takes acetate’s electrons in E1 active site
lipoamide
Lipoamide
Takes acetate’s E+ and transfer acetate to CoA in E2 active site
Where are electrons(E2) transferred to FAD?to make?
in E3 active site; FADH2
In dehydrogenase (E3) FADH2 transfers electrons to
NAD+
Can FADH2 go into the ETC
no
2 regulatory enzyme of PDH
pyruvate dehydrogenase kinase
pyruvate dehydrogenase phosphotase
PDH kinase
puts Pi on E1 active site (SLOWS PDH)
PDH phosphotase
takes Pi off E1 active site (PDH GO)
What are 2 ways to regulate PDH
competitive inhibition
kinase/phosphotase
When kinase is active (4)
SLOWS PDH high concentration of ATP lipoamide is reduced high concentration of NADH high concentration of Acetyl CoA
When phosphotase is active (3)
PDH GO
high concentration of ADP/AMP
high concentration of Mg+2
high concentration of Ca+2
