Lecture 10.22.14 (Exam #3)

Question 1

Limitations as to why PFK is the committing/rate determining step? (6)

Answer 1

ATP--slow
ADP/AMP--go
PEP-- no (feedback inhibition)
pH dec--no (ATP bind better)
F26BP--go
citrate--no

Question 2

TIM (3)

Answer 2

catalytically perfect
acid base catalysis
ketose–> aldose

Question 3

DHAP gets converted into

Answer 3

GAP

Question 4

GAP gets converted into

Answer 4

1,3 BPG

Question 5

Carbons in GAP vs glucose

Answer 5

GAP glucose
1 3 or 4
2 2 or 5
3 1 or 6

Question 6

In Step 6 NAD+ is

Answer 6

oxidize to NADPH

Question 7

substrate level phosphorylation

Answer 7

when a molecule with higher phosphoryl transfer potential donate a Pi to a molecule with a lower phosphoryl transfer potential

Question 8

Which steps in glycolysis is a substrate level phosphorylation?

Answer 8

Step 7 (PGK) and Step 10 (PK)

Question 9

mutase

Answer 9

type of isomerase

Question 10

What type of intermediate does TIM have

Answer 10

enediol