Lecture 10.24.14 (Exam #3) Flashcards
TIM
converts DHAP to GAP although the cell prefers to have a high {DHAP}
GAPDH
converts GAP to 1,3 BPG
What is the mechanism of GAPDH?
destabilize the transition state (high energy)
make thioester
lowers activation energy
NAD+ acts as a _____ in GAPDH
coenzyme
What is used in GAPDH to make the phosphate bond?
inorganic phosphate
T/F PGK is a substrate level phosphorylation.
TRUE
Mutase
a type of isomerase (Pi before and after catalysis)
How is PK regulated?
ATP & alanine inhibit
F26BP
F26BP participates in the ______ of PK.
feed forward activation
The big three enzymes that are metabolically irreversible?
hexokinase, PFK, PK
If there are high concentrations of glucose in the liver….
insulin in the pancreas tells glycolysis CAN happen
When there are high concentrations of glucose in the liver there is more (3)
copies of glucokinase
more F26BP
more copies of PK (PK w/o phosphate)
If there are low concentrations of glucose in the liver…..
glucagon in the pancreas tells glycolysis CAN NOT happen
When there are low concentrations of glucose in the liver there is less (3)
copies of glucokinase
less F26BP
less copies of PK (PK with phosphate)
What is the purpose of fermenation (2)
to keep glycolsis going without the presence of oxygen
to supply GAPDH with NAD+
What enzyme drives pyruvic acid to lactic acid
lactate dehydrogenase
in the heart;_____-> ______
in the muscle;_____ -> _____
LA to PA
PA to LA
Lactic Acid is made up of ____ monomers
4 tetramers of H&M
Where does the Cori Cycle begin?
in the muscle
(Cori Cycle) In the muscle; glucose is converted to ___ then ___
PA to LA
(Cori Cycle) After LA is produced in the muscle what happens
LA is sent to the liver via the bloodstream
(Cori Cycle) LA is converted to pyruvic acid and then back to glucose via
glucogenesis
