Lecture 1-3

Question 1

What is the prominent feature in Megakaryocyte Stage III

Answer 1

Multilobulated nuclei

Question 2

what stage where alpha and dense granules have fully developed and demarcation membrane system is also fully developed?

What is the color of the granules seen in this stage

Answer 2

Megarkaryocyte Stage III/Megakaryocyte

reddish-blue granules

Question 3

process where a single megakaryocyte shed platelets

how many platelets where shed by a single megakaryocyte

Answer 3

thrombopoiesis

2000-4000

Question 4

Where does the platelet released before it reaches circulation?

Describe the cell stated above.

Answer 4

via sinuses

Megakaryocyte with platelet budding

Question 5

prominent feature for megakaryoblast

Answer 5

plasma membrane bleb

Question 6

What stage where in it reaches its full ploidy level

Answer 6

End of Promegakaryocyte or MK-II stage

Question 7

T OR F. Megakaryocyte is adjacent to the membrane of the sinusoid lining endothelial cell

Answer 7

True

Question 8

TPO receptor site present st all maturation stages

Answer 8

MPL

Question 9

seen in early stages, a stem cell and common myeloid progenitor marker which disappears as differentiation proceeds

Answer 9

CD34

Question 10

What techniques that can detect cytoplasmic coagulstion factors and uses tossue samples

Answer 10

Immunostaining

Question 11

Techniques that uses antibodies that are directed against particulsr markers such as CD61

Answer 11

immunohistochemistry

Question 12

naked megakaryocyte nuclei are left behind and consumed by?

Answer 12

marrow macrophage

Question 13

primsry source of the impt. hormone in megakaryocytopoiesis

Answer 13

Liver

Question 14

acts in synergy in TPO to induce the early differentiation of stem cells

Answer 14

IL-3

Question 15

help enhance endomitosis, megakaryocyte maturation and thrombocytopoiesis

Answer 15

IL-11

Question 16

What are the substance that keeps things in check so that there arent too many megakaryovytes produce

Answer 16

platelet factor ,r
Beta-thromboglobulin
neutrophil-activsting peptide 2
interleukin 8

Question 17

Where does the platelet originate

Answer 17

megakaryocyte cytoplasm from bone marrow

Question 18

unique form of mitosis that occurs in megakaryocytic cells resukting in continues DNA reduplication without telophase and cytokines

Answer 18

Endomitosis

Question 19

Life span of platelets

Answer 19

9.5 days

Question 20

T or F. MPV as a predictor of mortality and cardiovascular risk

What is the MPV range for platelet

Answer 20

TRUE

8-10 femtoliter

Question 21

Markedly larger than ordinary platelets

What does it carry?

Answer 21

Reticulsted platelets or Stress platelets

free ribosimes and fragments of rough endoplasmic reticulum

Question 22

Reticulated platelets is associated with what risk?

Answer 22

increased risk of cardiovascular disease

Question 23

A platelet cytoskeleton that mainstains the platelet’s discoid shape

Answer 23

Microtubules

Question 24

A platelet activstion pathway that ends with the production of prostacyclin

Answer 24

Elcosanoid Pathway

Question 25

What enzyme responsible for digedting “unused” vWF

what is its other name

Answer 25

ADAMTS-13

vWF-cleaving protease

Question 26

Peptide sequence present in fibrinogen that mediates the binding of GP IIb/IIIa

Answer 26

Arginine-Glycine-Aspartic Acid

Question 27

antithrmbotic drug

Answer 27

Aspirin

Question 28

The modt commonly employed ways of determining platelet quantity

Answer 28

automated methods

Question 29

What techniques that provide s rapid estimstion of platelet numbers

Answer 29

Peripheral blood smears

Question 30

Normally, how many platelets seen per oil immersion field

Answer 30

8-20 platelets

Question 31

T OR F. at least 5 different OIFs shoukd be carefully examined for platelet estimation

Answer 31

False. It should be at least 10 OIF

Question 32

What is multiplied by the average number in 10 fields to arrive an approximation of the quantitative platelet concentration

Answer 32

20,000

Question 33

What quantitative method that determines the platelet count and uses 1% ammonium oxalate

Answer 33

Brecker-cronkite manual method

Question 34

The degree od clot retraction is directly proportional to the number of ________ and inversely proportional to the ______ and _______

Answer 34

number of platelet
hematocrit
level of fibrinogen