Laboratory: Clotting Time, PT and APTT

Question 1

What specific composition that help increase negativity of glass test tube

Answer 1

Chloride

Question 2

What are the main laboratory tests that measures coagulation and hemostasis

Answer 2

Activated Partial Thromboplastin Time and Prothrombin Time

Question 3

Incubate the 3 test tubes in what degree

Answer 3

37 C waterbath

Question 4

What laboratory procedures that Evaluates the intrinsic and common pathways of coagulation and monitors the effectiveness of heparin anticoagulant therapy

Answer 4

Clotting time

Question 5

Laboratory procedure that Evaluates the inherited & acquired deficiencies of the extrinsic and common pathways and Monitors the effectiveness of oral anticoagulant therapy

Answer 5

Prothrombin Time (PT)

Question 6

What anticoagulant used for prothrombin time

Answer 6

3.2 sodium citrate (0.105 – 0.109 M)

Light blue-topped evacuated tube

Question 7

What is the stimulus of the intrinsic pathway in the Lee and White method for clotting time test?

Answer 7

glass test tube

Question 8

What is the principle of the APTT and PT tests

Answer 8

clot-based testing procedures

Question 9

B1: What are the three methods used during clotting time?

B2: What is routinely used method during clotting time?

Answer 9

B1: Slide Metohd/Cappillary tube method/ Lee and White Method

B2: Lee and white method

Question 10

Name the two coagulation tests that monitors heparin anticoagulant therapy

Answer 10

Activated partial thromboplastin time, Clotting time

Question 11

B1: Recommended and standard methods in reporting PT

B2: what is the least used metohds in reporting PT

Answer 11

B1: International Normalized Ratio

B2: Percent Activity

Question 12

What is the composition of APTT reagent 2?

Answer 12

Calcium chloride

Question 13

B1: Substance used to moisten interior wall of the test tube

B2: Reason for adding the subtance

Answer 13

B1: Normal Saline SOlution

B2: increase negativity of glass test tube

Question 14

Specimen of choice used for prothrombin Time

Answer 14

Citrated platelet-poor plasma

Question 15

What laboratory procedure that Evaluates the inherited & acquired deficiencies of the intrinsic and common pathways, Monitors the effectiveness of heparin anticoagulant therapy, Detects the presence of circulating anticoagulant or inhibitor

Answer 15

Activated Partial Thromboplastin Time (APTT)

Question 16

Laboratory procedures that monitors the effect of oral anticoagulant therapy and is most sensitive to what deficiency

Answer 16

Factor VII deficiency

Question 17

What are the negatively charged substances that will activate the contact factor group

Answer 17

Silica, Celite, Kaolin, Elagic Acid

Question 18

Reagents used for APTT

Answer 18

Partial Thromboplastin Reagent

Calcium Chloride Reagent

Question 19

Specimen of Choice for APTT

Answer 19

citrated platelet-poor plasma

Question 20

APTT can measure all coagulation factors except:

Answer 20

Factor VII and Factor XIII

Question 21

APTT reagent is composed of:

Answer 21

phospholipids and negatively charged activators

Question 22

What is the normal reference range of the coagulation test that evaluates the intrinsic and common pathways and detects the presence of lupus anticoagulant?

Answer 22

25 to 35 seconds

Question 23

Reagent used in measuring the inherited and acquired deficiencies of the extrinsic and common pathway

Answer 23

Thromboplastin-Calcium Chloride Reagent

Question 24

State the Usefulness of Clotting Time

Answer 24

It measures the intrinsic and common pathway of coagulation

it monitors the effectiveness of heparin anticoagulant therapy