Hema Lec #5: Lab Evaluation of Coag Flashcards
Principle of most hemostasis/Coagulstion test
Clot-based testing procedure
All laboratory test in coagulation must incubate in what degree
37 degree Celsius waterbath
What will happen if blood is collected in a plastic tube but left standing at room temperature for a long period of time
destroyed thermal labile coagulation factors especially Factor VIII:C/destruction of Factor VIII:C
Bpank 1:What factor can be released if there is excessive probing during venipuncture?
Blank 2: The factor released will lead to the activation of what Specific coag system?
Blank 1: Tissue factor
B2: Activation of Extrinsic Pathway
Why do we need to collect the secone or third evacuated tube after a non additive in bet wen them
To avoid potential carry over contamination of previous anticoagulants
Anticoagulant of choice for most hemostasis testing
3.2% sodium citrate
What is the action of the anticoagulsnt of choice for most hemostasis testing
chelates the calcium to prevent coagulation
Prolonged tourniquet application could cause:
Hemoconcentration
Increased fibrinolytic activity
Sodium Citrate preserves labile coagulation factors IV and VIII
False
What specimen is obtained after centrifuged and is the spx of choice for clot-based testing procedure
for how many minutes of spinning and specific gravity for this specimen to obtain
Citrated Platelet-Poor Plasma
15-20 minutes at 2500 gravity
What studies used in Citrated Platelet-Rich Plasma
How many minutes required for the centrifugae and gravity to obtain such specimen
Platelet Aggregation Studies OR Platelet Aggregometry
10 minutes; 200 gravity
For how many hours does the Citrated platelet-rich plasma needed to store?
Room temperature for 3 hrs.
if prolonged, discard and another sample is collected 🤪🤪
Principles of Clotting Time: Time required for the blood to clot. What are the sub-principles that supports the principle of clotting tome
After the blood was taken out from the vessels
Upon or after the activation of coagulstion factors
Usefullness of Clotting Time
It measures the intrinsic and common pathways of coagulstion
It monitors the effectiveness of heparin anticoagulant therapy
Primary usefulness of clotting time
It measures the intrinsic and common pathways of coagulation
Monitors the effectiveness of the heparin anticoagulant therapy
routine used method in clotting time procedure
Lee and White Method
Substance used to moiten the integral part of the glass tt
Purpose:
Normal Saline Solution
to increase negativity of glass tt
What composition of NSS that will help the glass tt increase its negativity
Chloride
Specimen used for lee and white method
Whole blood
The main laboratory tests that measures coagulation and hemostasis
Activated Partial Thromboplastin Time AND
Prothrombin Time
Laboratory test that measures the effect of heparin therapy and to detect circulating anticoagulant
Activated Partial Thromboplastin Time
What is the principle for APTT
What is its normal value
Clot-based testing procedure/ Clot-based test
25-35 seconds
Usefulness of APTT
It measures the inherited AND acquired deficiency in the intrinsic and common pathway
It monitors the effectiveness of heparin anticoagulant therapy
It detects the presence of circulating anticoagulant
2 reagents in APTTA
Reagent 1: Partial Thromboplastin Reagent
Reagent 2: Calcium chloride Reagent
