Lab Safety Flashcards
Health Safety at Work etc Act _____
You must work safely
You must not endanger others
You must not misuse safety equipment
Penalty – up to _____ in prison &/or an unlimited fine
1974
2 year
The Management of Health and Safety at Work Regs ___
Control of Substances Hazardous to Health Regs _____
= You must perform \_\_\_\_\_\_\_\_
1999
2004
RISK ASSESSMENTS
All diagnostic and health care laboratories must be designed and organized for Biosafety level __ or above
2
About the premises
(Low or high?) ceiling with good ventilation
for walls and ceiling use _______,_____ paint
High
washable, glossy
Bench tops
______ covering, easy to clean, resistant to chemicals and disinfectants
no _____, no ____
non-porous
wood; steel
Who is responsible for the lab’s safety management?
Safety officer
Control Measures
Use a (more or less?) risky substance
Use a safer form of that substance (eg ______ instead of _______)
Less
solution; powder
Control Measures
Totally enclose the process (eg a _______)
Partially enclose the process (eg with a ______)
Ensure good general ventilation
glove-box
fume cupboard
Liquid gases are extremely _____ and can cause _____
Liquid gases evaporate and many can cause _____
cold
Burns
asphyxiation
________________ (PPE)
Personal Protective Equipment
Steps in hazards exposure
_____________ of specimens
Sample _____
Laboratory chemicals & electrical hazards
______ sample & other materials
Collection & transport
processing
Discard
Sample processing
____ before handling specimen
Careful __________
Use of ________
Proper use of equipments like Loop, Lamp, Centrifuge
________ after finishing work
PPE
opening & closing of container.
safety cabinet
Hand washing
Hazard Groups
Group1. organisms most likely to _____
not cause human disease
Hazard Groups
Group 2. organisms may ________ and _________ but _____________
cause human disease & hazards to lab workers
unlikely to spread in community
Hazard Groups
Group 3. organisms may ________, __________ & _________ but _________
cause severe human disease
presents a serious hazard to workers
pose a risk to spread in community
there is effective treatment or prophylaxis
Hazard groups
Group 4.
organisms may _________,________, and ________ & there is __________
cause severe human disease
presents a serious hazard to workers
pose high risk to spread in community
no effective treatment or prophylaxis
Containment levels
Containment Level 1 laboratory – working with organisms from hazard group ___ and ______ care to be taken
1
no special
Containment levels
pathogens fall in hazard ___ & must be handled in containment level 2 labs
They are (Easy or Difficult ?) to clean with adequate _____, Limited ____ , ____ and ____
_____ for treatment & disposal of waste
2; Easy
space; access
Gowns & gloves
Autoclave;
Containment level 2 labs use a Class _____ microbiological safety cabinet
1 or 2
Containment level 3
work with category ___ hazard pathogens
______ area with ______ sign
_______ access
______ ventilation & ___ filters
Own set of incubators, centrifuge, refrigerators
3
Isolated; biohazard
Restricted; Continuous; HEPA
Containment level 3 labs use a Class _________ safety cabinets
2 or class 3
In containment level 3 labs, Gowns may or may not be autoclaved after use
T/F
F
It must be autoclaved
Containment level 4
hazard group __
_____ area with ____ zone
______ warning signs & instructions
Sophisticated control of air movement
Regular _______ of equipments like incubators & safety cabinets
Regular ______
4
Isolated; buffer
Biohazard
disinfection
fumigation
_______ is the strictest containment category
Containment level 4
_____ containment level requires the Full PPE gear with respirators
Containment level 4
Containment level 4 labs Use sealed class _______ safety cabinets
3
Disinfection of cabinets
We have ____ methods
2
A and B
Disinfection of cabinets
Method A – use of __ ml _____ (___% _____) with use of _____ •
25
formalin
40; formaldehyde
vaporizer
Disinfection of cabinets
Method B – use of ___ ml ____ + __ g of ________
Then Cabinets is sealed for _______ or ______
Warning sign placed over it
Then ________ & slightly open the ______
35; formalin
10; potassium permanganate
overnight or at least for 5 hrs.
Switch on the fan
front pannel
Disinfection of incubators
By using ________
Methyl alcohol
prophylaxis
__________ to ______
treatment given or action taken to prevent disease
PEP (-_____________)
post exposure prophylaxis
For disinfecting due to spillages
Hypochlorites (__%) – weak ____ p.p.m. available Cl2 for routine surface disinfection
Hypochlorites (___%) – weak ___ p.p.m. available Cl2 for blood spills & virology
______(__%) for skin disinfection
I ; 1000
10; 10000
Alcohol; 70
Disposal of laboratory material
Blood and body fluids (__________ waste) should be Treated with _____ or ________ and put down the _________. •
Regulated medical
bleach or autoclaved
Sanitary sewer
Disposal of laboratory material
Microbiological Waste including Biosafety Level ______ organisms: (Regulated medical waste) should be ______ at _____ °C. or ______ treated and put down ______.
1, 2 and 3
Autoclaved; 121; chemically
sanitary sewer
Autoclaves are tested (daily,weekly or monthly ?) using bio indicators _____________ ___________
Weekly
Geobacillus stearothermophilus
Disposal of laboratory material
Laboratory solid waste – collected & transported to __________ in proper ________
hospital waste management
color coded bags
