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House Clinical Pathology > Lab Safety > Flashcards

Lab Safety Flashcards

1
Q

Health Safety at Work etc Act _____

You must work safely
You must not endanger others
You must not misuse safety equipment

Penalty – up to _____ in prison &/or an unlimited fine

A

1974

2 year

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2
Q

The Management of Health and Safety at Work Regs ___
Control of Substances Hazardous to Health Regs _____

  = You must perform \_\_\_\_\_\_\_\_
A

1999

2004
RISK ASSESSMENTS

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3
Q

All diagnostic and health care laboratories must be designed and organized for Biosafety level __ or above

A

2

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4
Q

About the premises

(Low or high?) ceiling with good ventilation
for walls and ceiling use _______,_____ paint

A

High

washable, glossy

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5
Q

Bench tops
______ covering, easy to clean, resistant to chemicals and disinfectants
no _____, no ____

A

non-porous

wood; steel

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6
Q

Who is responsible for the lab’s safety management?

A

Safety officer

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7
Q

Control Measures

Use a (more or less?) risky substance
Use a safer form of that substance (eg ______ instead of _______)

A

Less

solution; powder

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8
Q

Control Measures

Totally enclose the process (eg a _______)
Partially enclose the process (eg with a ______)
Ensure good general ventilation

A

glove-box

fume cupboard

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9
Q

Liquid gases are extremely _____ and can cause _____
Liquid gases evaporate and many can cause _____

A

cold
Burns

asphyxiation

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10
Q

________________ (PPE)

A

Personal Protective Equipment

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11
Q

Steps in hazards exposure

_____________ of specimens

Sample _____

Laboratory chemicals & electrical hazards

______ sample & other materials

A

Collection & transport

processing

Discard

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12
Q

Sample processing

____ before handling specimen
Careful __________
Use of ________
Proper use of equipments like Loop, Lamp, Centrifuge
________ after finishing work

A

PPE

opening & closing of container.

safety cabinet

Hand washing

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13
Q

Hazard Groups

Group1. organisms most likely to _____

A

not cause human disease

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14
Q

Hazard Groups

Group 2. organisms may ________ and _________ but _____________

A

cause human disease & hazards to lab workers

unlikely to spread in community

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15
Q

Hazard Groups

Group 3. organisms may ________, __________ & _________ but _________

A

cause severe human disease

presents a serious hazard to workers

pose a risk to spread in community

there is effective treatment or prophylaxis

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16
Q

Hazard groups

Group 4.

organisms may _________,________, and ________ & there is __________

A

cause severe human disease

presents a serious hazard to workers

pose high risk to spread in community

no effective treatment or prophylaxis

17
Q

Containment levels

Containment Level 1 laboratory – working with organisms from hazard group ___ and ______ care to be taken

A

1

no special

18
Q

Containment levels

pathogens fall in hazard ___ & must be handled in containment level 2 labs

They are (Easy or Difficult ?) to clean with adequate _____, Limited ____ , ____ and ____

_____ for treatment & disposal of waste

A

2; Easy

space; access

Gowns & gloves

Autoclave;

19
Q

Containment level 2 labs use a Class _____ microbiological safety cabinet

A

1 or 2

20
Q

Containment level 3

work with category ___ hazard pathogens

______ area with ______ sign

_______ access

______ ventilation & ___ filters

Own set of incubators, centrifuge, refrigerators

A

3

Isolated; biohazard

Restricted; Continuous; HEPA

21
Q

Containment level 3 labs use a Class _________ safety cabinets

A

2 or class 3

22
Q

In containment level 3 labs, Gowns may or may not be autoclaved after use

T/F

A

F

It must be autoclaved

23
Q

Containment level 4

hazard group __

_____ area with ____ zone

______ warning signs & instructions

Sophisticated control of air movement

Regular _______ of equipments like incubators & safety cabinets

Regular ______

A

4

Isolated; buffer

Biohazard

disinfection

fumigation

24
Q

_______ is the strictest containment category

A

Containment level 4

25
Q

_____ containment level requires the Full PPE gear with respirators

A

Containment level 4

26
Q

Containment level 4 labs Use sealed class _______ safety cabinets

A

3

27
Q

Disinfection of cabinets

We have ____ methods

A

2

A and B

28
Q

Disinfection of cabinets

Method A – use of __ ml _____ (___% _____) with use of _____ •

A

25

formalin

40; formaldehyde

vaporizer

29
Q

Disinfection of cabinets

Method B – use of ___ ml ____ + __ g of ________

Then Cabinets is sealed for _______ or ______

Warning sign placed over it

Then ________ & slightly open the ______

A

35; formalin

10; potassium permanganate

overnight or at least for 5 hrs.

Switch on the fan

front pannel

30
Q

Disinfection of incubators

By using ________

A

Methyl alcohol

31
Q

prophylaxis

__________ to ______

A

treatment given or action taken to prevent disease

32
Q

PEP (-_____________)

A

post exposure prophylaxis

33
Q

For disinfecting due to spillages

Hypochlorites (__%) – weak ____ p.p.m. available Cl2 for routine surface disinfection

Hypochlorites (___%) – weak ___ p.p.m. available Cl2 for blood spills & virology

______(__%) for skin disinfection

A

I ; 1000

10; 10000

Alcohol; 70

34
Q

Disposal of laboratory material

Blood and body fluids (__________ waste) should be Treated with _____ or ________ and put down the _________. •

A

Regulated medical

bleach or autoclaved

Sanitary sewer

35
Q

Disposal of laboratory material

Microbiological Waste including Biosafety Level ______ organisms: (Regulated medical waste) should be ______ at _____ °C. or ______ treated and put down ______.

A

1, 2 and 3

Autoclaved; 121; chemically

sanitary sewer

36
Q

Autoclaves are tested (daily,weekly or monthly ?) using bio indicators _____________ ___________

A

Weekly

Geobacillus stearothermophilus

37
Q

Disposal of laboratory material

Laboratory solid waste – collected & transported to __________ in proper ________

A

hospital waste management

color coded bags

House Clinical Pathology (45 decks)

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