Lab quiz 4 Flashcards

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1
Q

what is a gram stain

A

result based on properties of cell wall

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2
Q

what does gram positive look like

A

purple

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3
Q

what does gram negative look like

A

red

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4
Q

what is the procedure for a differential stain

A

crystal violet- 1 min
wash
iodine- 1 min
wash
alcohol- 5 sec
wash
safronin- 1 min
wash
blot

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5
Q

what is the initial stain

A

crystal violet

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6
Q

what is the mordant

A

iodine

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7
Q

what is the decolorizer

A

alcohol

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8
Q

what is the counter stain

A

safronin

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9
Q

what would dead bacteria appear as

A

gram neg

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10
Q

what do clumps appears as

A

gram pos

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11
Q

what does too much alc appear as

A

gram neg

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12
Q

what does too little alc appear as

A

gram pos

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