Lab quiz 3

Question 1

what are simple stains for

Answer 1

increasing refractive index
morphology

Question 2

whta kind of charge do bacteria (DNA, membrane, wall…) have

Answer 2

slight negative charge

Question 3

what kind of charge does the environment have

Answer 3

slight positive charge

Question 4

what are in stains

Answer 4

salts
chromophone
and ions

Question 5

how to prepare a smear

Answer 5

sterilize loop
transfer loop of water to slide
sterilize loop
transfer and suspend a small amoutn of bacteria to water on slide
spread out suspension and air dry
heat fix 3 passes
stain flood smear for 1 minute
wash
blot