Lab Math Flashcards

1
Q

Calculation for Dilution

A

V1C1=V2C2 remember to subtract or add for the total volume when asking for quantity to dilute.

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2
Q

1 inch = how many cm

A

2.54cm

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3
Q

1 kg = how many lb

A

2.2 lb

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4
Q

RHIG formula

A

% of fetal cells/ mother cells multiply by 5000mL= FMH quantity/30 (30uL is covered by any one Rhig vial) To this final number at 1 vial to be on the safe side

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5
Q

Exchange transfusion (Mixing plasma and RBCs)

A

V1C1=V2C2 remember to subtract or add for the total volume when asking for quantity to dilute. Final volume is total final volume, not the diluent.

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6
Q

Antigen screening calculation

A

% Ag Neg/100=Units needed/X units to screen. Units needed/%antigen negative

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7
Q

Blood volume calculation

A

convert lbs to Kg. 1kg=75ml/Kg of blood this is the blood volume

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8
Q

Plasma volume

A

Blood volume (hct%)=X, BV-X= plasma volume. BV(1-HCT)

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9
Q

Maximum blood bolume collected according to AABB

A

10.5mL/ Kg, don’t forget 50mL diversion pouch

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10
Q

Component yield

A

Total plt= (Plt/uL) (10^3uL/mL)(vol in unit). PC/WB=% of yield. With a WB unit you do the count of the WB unit and the count of the PC (platelet concentrate and then take a percentage)

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11
Q

Average yield of a platelet unit

A

69%

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12
Q

Half life of IgG

A

23-25 days

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13
Q

Cryo dosing (Factor VIII)

A

(PV)(Factor VIII increment)/ 80IU per bag

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14
Q

Factor VIII quantity in severe hemophilia

A

Assume 0%

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15
Q

normal Factor 8 quantity in cryo bag

A

80IU/bag

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16
Q

Corrected Count Increment

A

(New ct-Old ct) (BSA)/(Plt)(x10^11)

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17
Q

Average BSA if not given

A

2m^2

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18
Q

successful vs. unsuccesful platelet recovery (CCI)

A

> 7500 successful <7500 on two subsequent transfusions= refractory.

19
Q

Plasma exchange

A

the same as plasma volume really, just depends on how many times you want to exchange the patients plasma and multiply that but the final number. BV(1-hct) =PE

20
Q

Hardy Weinberg Equilibrium

A

p+q=1, p^2+2pq+q^2=1. q^2 = percentage of homozygous negative- use this as the initial portion of the calculation

21
Q

RR

A

HLA and disease correlation. Draw a graph is the first step and plug the numbers into the graph. (Positive/Positive#)x(Negative/Negative#)/ (Pos/Neg#)(Neg/Pos#)

22
Q

RCF

A

relative centrifugal force=(28.38 or 11.17)(r)(rpm/1000)^2

23
Q

Sensitivity

A

TP/(TP+FN)

24
Q

Specificity

A

TN/(TN+FP)

25
Q

RCF incheas value

A

28.38

26
Q

RCF cm value

A

11.17

27
Q

FTE formula

A

determine the acutal time worked per person (deduct vacation sick days etc). - factor in productivity (in a percentage) convert all units to min/yr. FTE= annual test workload (always provided)/annual workload (productivity and vacation info provided)

28
Q

Staffing budgeting compare cost

A

determine quantity of test (min/year)/ annual workload (minutes/year) this is the total FTE needed, compare the FTE and salary vs. the cost of the test/year to determine which saves more money

29
Q

Requirement for fibrinogen in cryo unit

A

> /=150

30
Q

half life of IgG

A

21-28 days

31
Q

Half life of IgM

A

5 days

32
Q

Half life of factor VII

A

5 hours

33
Q

Half life of factor VIII

A

8-12 HOURS

34
Q

Half life of Factor X

A

20 HOURS

35
Q

calulating yield of cryo

A

units of factor 8/mL x mL of final product divided by (units of factor 8/mL x mL of original FFP product) = percentage

36
Q

1 plasma volume exchange removes approximately how much of material?

A

65%

37
Q

calculating the allowed amount in donors under 110lb

A

450(normal amount of blood allowed)x donor weight lb .110lb= amount left

38
Q

amount of anticoagulant used

A

450mL +/- 10% =63 mL anticoagulant. 500mL +/- 10% = 70mL

39
Q

recalculate the amount of anticoagulant needed for donor unit when patient is less than 110 lb

A

63mL/110lb=amount needed (mL)/donor weight (lb)

40
Q

how much fibrinogen is in one bag of cryo

A

250 mg/ bag

41
Q

BV for IUT

A

0.14 mL/g

42
Q

IUT calculation

A

(fetus weight in grams) (0.14mL/g)(desired change in hematocrit) / hct of unit to transfuse

43
Q

quantity of one reagent drop used in blood testing

A

50uL

44
Q

formula for conversion celsius to farenheit

A

T in celsius= (Tin farenheit-32) x 5/9