LAB MANAGEMENT (Laboratory Safety) Flashcards
ENUMERATE HIERARCHY OF CONTROLS FROM MOST TO LEAST EFFECTIVE
ELIMINATION
SUBSTITUTION
ENGINEERING CONTROLS
ADMINISTRATIVE CONTROLS
PPE
hierarchy of controls that physically removes the hazard
elimination
hierarchy of controls that replaces the hazard
substitution
hierarchy of controls that isolate people from the hazard
engineering controls
hierarchy of controls that changes the way people work
administrative controls
hierarchy of controls that protect the workers with protective eq
PPE
Hierarchy of controls:
Removes the hazard at the source
ELIMINATION
Hierarchy of controls:
Preferred solution to protect workers because no exposure can occur.
ELIMINATION
Hierarchy of controls:
Using a safer alternative to the source of the hazard.
SUBSTITUTION
Hierarchy of controls:
Reduce potential for harmful effects, no new risks.
SUBSTITUTION
Hierarchy of controls:
Reduce or prevent hazards from coming into contact with workers
ENGINEERING
CONTROLS
Hierarchy of controls:
Include modifying equipment or the workspace, using protective barriers, ventilation, and more.
ENGINEERING
CONTROLS
Hierarchy of controls:
Cost more than administrative controls or PPE. However, long-term operating costs are lower, especially when protecting multiple workers.
ENGINEERING
CONTROLS
Hierarchy of controls:
Can save money in other areas of the work process or facility operation.
ENGINEERING
CONTROLS
Hierarchy of controls:
Establish work practices that reduce the duration, frequency, or intensity of exposure to hazards.
ADMINISTRATIVE CONTROLS
Included in ADMINISTRATIVE CONTROLS
- Work process training
- Ensuring adequate rest breaks
- Limiting access to hazardous areas or machinery
- Adjusting line speeds
- Job rotation
Hierarchy of controls:
Equipment worn to minimize exposure to hazards. Less expensive than other controls, but can be costly over time.
PPE
Addressed in PPE program
- Workplace hazards assessment
- Inspection and replacement of damaged or worn-out PPE
- PPE selection and use
- Program monitoring for continued effectiveness
- Employee training
Disease producing agents (pathogens) that can be transmitted to individuals through various routes of exposure (modes of transmission). Exposure to these hazards may result in acute or chronic health conditions.
BIOLOGICAL HAZARDS
Pathogens that can be transmitted through:
Blood and body fluids
- HBV
- HCV
- HIV
Pathogens that can be transmitted through:
Fecal-Oral
- HAV
- HEV
Pathogens that can be transmitted through:
Contact
- HSV
- HPV
- CMV
- Vancomycin Resistant Enterococcus (VRE)
- Methicillin Resistant Staphylococcus Aureus
(MRSA) - Clostridium difficile (C. difficile)
Pathogens that can be transmitted through:
Small particle aerosol (airborne)
- Mycobacterium Tuberculosis
Pathogens that can be transmitted through:
>1 mode: contact, and/or droplet spray
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
- Herpes Zoster Virus (HZV)
- Influenza virus (Flu)
- Measles (Rubeola virus)
Bacteria, virus, fungi, parasite
Pathogens/Infectious agents
Habitat for organism to grow:
human, animal, air, food, soil, water, equipment
Reservoir
Secretions (saliva)
Excretions (urine, feces)
Portal of exit
Contact, droplet, airborne, vector, fomites
Mode of transmission
Mucosa lining, open wound, oral, respiratory tract, urinary tract
Portal of entry
Very young and elderly, those with chronic disease, malnourished, immunocompromised
Susceptible host
Contains circles representing the chain of infection
BIOHAZARD SYMBOL
3 circles represented in biohazard symbol
- Host
- Agent
- Transmission
BSL with NO known potential for infecting healthy people.
BSL1
BSL associated with rarely serious human diseases.
Preventive/therapeutic interventions are often available.
BSL 2
BSL for viruses not normally encountered and mycobacteria
BSL3
(with risk-based enhancements)
BSL for dangerous and exotic agents that pose higher risk of aerosol-transmitted laboratory infections and life-threatening disease.
Effective treatments are limited.
BSL4
FEATURES OF BSL1
- Controlled access
- Hand washing sink
- Sharp hazards warning policy
- PPE
- Laboratory bench
- Autoclave
FEATURES OF BSL 2
- Controlled access
- Hand washing sink
- Sharp hazards warning policy
- Physical containment device
- PPE
- Laboratory bench
- Autoclave
FEATURES OF BSL3
- Self-closing, double-door access
- Controlled access
- Personal shower out
- Sharp hazards warning policy
- Hand washing sink
- Sealed penetrations
- Physical containment device
- Powered air purifying respirator
- Laboratory bench
- Autoclave
- Exhaust HEPA filter
- Effluent decontamination system
- Air tight when disinfecting
FEATURES OF BSL4
- Air tight
- Self-closing, double-door access
- Controlled access
- Sharp hazards warning policy
- Hand washing sink
- Sealed penetrations
- Physical containment device
- Positive pressure protective suit
- Laboratory bench
- Autoclave
- Chemical shower out
- Personal shower out
- Supply and exhaust HEPA filter
- Effluent decontamination system
Most important means of preventing the spread of infection
HAND WASHING
Duration for handwashing accdg. to CDC
20 secs
rinsed downward
Performed if hands are visibly soiled
wash hands with soap and water for 1-2 mins
Performed if hands are NOT visibly soiled
wash hands for at least 30 secs. or
use alcohol-based hand cleaners
Worn to protect clothing and skin
Protect workers from contamination
Laboratory gown
Worn to protect against splashes and inhalation of droplets containing pathogens from infective px.
Masks, goggles, face shields
Worn to protect healthcare workers from contamination by px body substances.
Gloves
Correct sequence of DONNING
- Gown
- Mask (covers both nose & mouth)
- Gloves (pulled over the gown cuff)
Correct sequence of DOFFING
- Gloves
- Gown (pulled from shoulders towards the hands)
- Mask
Used substances for decontamination of body fluid spills
5.25% NaOCL or
10% chlorine bleach (1:10 dilution)
Recommended contact time for decontamination of body fluid spills
15 minutes
Under health hazard
- Carcinogen
- Mutagenicity
- Reproductive toxicity
- Respiratory sensitizer
- Target organ toxicity
- Aspiration toxicity
Under flame hazard
- Flammables
- Pyrophorics
- Self-heating
- Emits flammable gas
- Self-reactives
- Organic peroxides
Under exclamation mark hazard
- Irritant (skin and eye)
- Skin sensitizer
- Acute toxicity
- Narcotic effects
- Respiratory tract irritant
- Hazardous to ozone layer (non-mandatory)
Under gas cylinder hazard
Gases under pressure
Under corrosion hazard
- Skin corrosion/burns
- Eye damage
- Corrosive to metals
Under exploding bomb hazard
- Explosives
- Self-reactives
- Organic peroxides
Under flame over circle hazard
oxidizers
Under environment hazard (non-mandatory)
Aquatic toxicity
Under skull and crossbones hazard
Acute toxicity (fatal or toxic)
Communicate hazards associated with using chemicals
SAFETY DATA SHEETS (SDS)
Formerly: Material Safety Data Sheets (MSDS)
Important source of information for workers, lab personnel, emergency responders, and emergency medical personnel
SAFETY DATA SHEETS (SDS)
Formerly: Material Safety Data Sheets (MSDS)
Content of a Safety Data Sheet (16 sections)
- Identification
- Hazard identification
- Composition/Information on Ingredient
- First aid measures
- Fire fighting measures
- Accidental release measures
- Handling and storage
- Exposure controls/personal protection
- Physical and chemical properties
- Stability and reactivity
- Toxicological information
- Ecological information
- Disposal consideration
- Transport information
- Regulatory information
- Other information
PEL
Permissible Exposure Limits
TLV
Threshold Limit Values
T/F
Storage should not be based solely on alphabetical order because incompatible chemicals may react chemically.
T
Flammable liquid is stored separately from:
Flammable solids
Mineral acid is stored separately from:
Organic acids
Caustics is stored separately from:
Oxidizers
Perchloric acid is stored separately from:
Water-reactive substances
Air-reactive substances is stored separately from:
Others
Heat-reactive substances requiring refrigeration is stored separately from:
Others
Equivalent of 0 in hazard
Minimal
Equivalent of 1 in hazard
Slightly hazardous
Equivalent of 2 in hazard
Hazardous
Equivalent of 3 in hazard
Extremely hazardous
Equivalent of 4 in hazard
Dangerous
Color RED in NFPA standard hazard
Fire hazard
Color YELLOW in NFPA standard hazard
INSTABILITY OR REACTIVITY
Color BLUE in NFPA standard hazard
Health hazard
Color WHITE in NFPA standard hazard
Specific hazard
Fire hazard
4 -
3 -
2 -
1 -
0 -
4 - Below 73F
3 - Below 100F
2 - Below 200F
1 - Above 200F
0 - Will not burn
Instability
4 -
3 -
2 -
1 -
0 -
4 - May detonate
3 - Shock + Heat may detonate
2 - Violent chemical change
1 - Unstable if heated
0 - Stable
Health Hazard
4 -
3 -
2 -
1 -
0 -
4 - Deadly
3 - Extreme danger
2 - Hazardous
1 - Slightly hazardous
0 - Normal material
Specific hazards
OX -
ALK -
W -
SA -
ACID -
COR -
Rad symbol -
OX - Oxidizers
ALK - Alkaline
W - Use no water
SA - Simple Asphyxiant
ACID - Acid
COR - Corrosive
Rad symbol - Radioactive
Found at the long wavelength end of the spectrum and may have enough energy to excite molecules and atoms causing them to vibrate faster.
Non-ionising radiation
Type of radiation:
Radio, TV, microwave
Non-ionising radiation
Type of radiation in infrared region
Non-ionising radiation
Type of radiation with increasing wavelength
Non-ionising radiation
Has more energy; enough to cause chemical changes by breaking chemical bonds.
This effect can cause damage to living
tissue.
Ionising radiation
Type of radiation in ultraviolet region
Ionising radiation
Type of radiation: Medical X-ray, radioactive sources
Ionising radiation
Type of fire: Ordinary combustibles (wood, paper, cloth)
Class of fire:
Fire extinguishers:
Class of fire: A
Fire extinguishers: Type A, A-B
Type of fire: Flammable liquids (gasoline, paints, oils, grease)
Class of fire:
Fire extinguishers:
Class of fire: B
Fire extinguishers: Type A-B, B-C, A-B-C
Type of fire: Live electrical equipment (electrical wiring, fuse box)
Class of fire:
Fire extinguishers:
Class of fire: C
Fire extinguishers: Type B-C, A-B-C
Type of fire: Commercial cooking equipment
Class of fire:
Fire extinguishers:
Class of fire: K
Fire extinguishers: Wet chemical
Type of fire: Combustible metal
Class of fire:
Fire extinguishers:
Class of fire: D
Fire extinguishers: Bucket of sand
Activities characterized by constant/excessive repetitive actions, mechanical pressure, vibrations, compressive forces or static postures for an extended period which may cause cumulative trauma disorders or work-related MSD.
ERGONOMIC HAZARD
Give factors for ergonomic hazard
- Inadequate lighting or glare
- Awkward postures
- Forceful exertions
- Vibrations
- Contact stress
- Static postures
- Repetitive motions
Container color for non-infectious dry waste
Black
Container color for non-infectious wet waste (kitchen, dietary)
Green
Container color for infectious and pathological waste
Yellow
Container color for chemical waste including heavy metal
Yellow with black band
Container color for radioactive waste
Orange
Container color for sharps and pressurized containers
Red
represented by the circles contained within the biohazard symbol
chain of infection
BSL:
Nonpathogenic E. coli
1
BSL:
Gram neg, pos
S. aureus
P. aeruginosa
2
BSL:
M. tuberculosis
3
BSL:
SARS-CoV-2
Ebola
BSL 4
Unique feature of BSL 2 from BSL 1
physical containment device
risk based enhancements in BSL 3
personal shower out
powered air purifying respirator
exhaust HEPA filter
effluent decontamination system
Unique features in BSL 3 not seen in BSL 2
Self-closing, double door access
Personal shower out
Sealed penetrations
PAP respirator
Exhaust HEPA filter
Effluent decontamination system
BSL that is airtight when disinfecting
BSL 3
BSL that is airtight not only during disinfecting
BSL 4
Unique features of BSL 4 not seen in BSL 3
Positive pressure protective suit
Chemical shower out
Personal shower out
Factors that contributes to FIRE
Fuel
Heat
Oxygen
Chemical reaction
NFPA
National Fire Protection Association
Wastes that are delayed to decay
Chemical waste
Pharmaceutical waste
Radioactive waste
Types of waste that are BURIED after treatment by non-burn technologists
sharps
pathological waste
Types of waste that are collected and disposed by suppliers
aerosol/pressurized containers
pharmaceutical waste
radioactive waste