LAB MANAGEMENT (Laboratory Safety) Flashcards
ENUMERATE HIERARCHY OF CONTROLS FROM MOST TO LEAST EFFECTIVE
ELIMINATION
SUBSTITUTION
ENGINEERING CONTROLS
ADMINISTRATIVE CONTROLS
PPE
hierarchy of controls that physically removes the hazard
elimination
hierarchy of controls that replaces the hazard
substitution
hierarchy of controls that isolate people from the hazard
engineering controls
hierarchy of controls that changes the way people work
administrative controls
hierarchy of controls that protect the workers with protective eq
PPE
Hierarchy of controls:
Removes the hazard at the source
ELIMINATION
Hierarchy of controls:
Preferred solution to protect workers because no exposure can occur.
ELIMINATION
Hierarchy of controls:
Using a safer alternative to the source of the hazard.
SUBSTITUTION
Hierarchy of controls:
Reduce potential for harmful effects, no new risks.
SUBSTITUTION
Hierarchy of controls:
Reduce or prevent hazards from coming into contact with workers
ENGINEERING
CONTROLS
Hierarchy of controls:
Include modifying equipment or the workspace, using protective barriers, ventilation, and more.
ENGINEERING
CONTROLS
Hierarchy of controls:
Cost more than administrative controls or PPE. However, long-term operating costs are lower, especially when protecting multiple workers.
ENGINEERING
CONTROLS
Hierarchy of controls:
Can save money in other areas of the work process or facility operation.
ENGINEERING
CONTROLS
Hierarchy of controls:
Establish work practices that reduce the duration, frequency, or intensity of exposure to hazards.
ADMINISTRATIVE CONTROLS
Included in ADMINISTRATIVE CONTROLS
- Work process training
- Ensuring adequate rest breaks
- Limiting access to hazardous areas or machinery
- Adjusting line speeds
- Job rotation
Hierarchy of controls:
Equipment worn to minimize exposure to hazards. Less expensive than other controls, but can be costly over time.
PPE
Addressed in PPE program
- Workplace hazards assessment
- Inspection and replacement of damaged or worn-out PPE
- PPE selection and use
- Program monitoring for continued effectiveness
- Employee training
Disease producing agents (pathogens) that can be transmitted to individuals through various routes of exposure (modes of transmission). Exposure to these hazards may result in acute or chronic health conditions.
BIOLOGICAL HAZARDS
Pathogens that can be transmitted through:
Blood and body fluids
- HBV
- HCV
- HIV
Pathogens that can be transmitted through:
Fecal-Oral
- HAV
- HEV
Pathogens that can be transmitted through:
Contact
- HSV
- HPV
- CMV
- Vancomycin Resistant Enterococcus (VRE)
- Methicillin Resistant Staphylococcus Aureus
(MRSA) - Clostridium difficile (C. difficile)
Pathogens that can be transmitted through:
Small particle aerosol (airborne)
- Mycobacterium Tuberculosis
Pathogens that can be transmitted through:
>1 mode: contact, and/or droplet spray
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
- Herpes Zoster Virus (HZV)
- Influenza virus (Flu)
- Measles (Rubeola virus)
Bacteria, virus, fungi, parasite
Pathogens/Infectious agents
Habitat for organism to grow:
human, animal, air, food, soil, water, equipment
Reservoir
Secretions (saliva)
Excretions (urine, feces)
Portal of exit
Contact, droplet, airborne, vector, fomites
Mode of transmission
Mucosa lining, open wound, oral, respiratory tract, urinary tract
Portal of entry
Very young and elderly, those with chronic disease, malnourished, immunocompromised
Susceptible host
Contains circles representing the chain of infection
BIOHAZARD SYMBOL
3 circles represented in biohazard symbol
- Host
- Agent
- Transmission
BSL with NO known potential for infecting healthy people.
BSL1
BSL associated with rarely serious human diseases.
Preventive/therapeutic interventions are often available.
BSL 2
BSL for viruses not normally encountered and mycobacteria
BSL3
(with risk-based enhancements)
BSL for dangerous and exotic agents that pose higher risk of aerosol-transmitted laboratory infections and life-threatening disease.
Effective treatments are limited.
BSL4
FEATURES OF BSL1
- Controlled access
- Hand washing sink
- Sharp hazards warning policy
- PPE
- Laboratory bench
- Autoclave
FEATURES OF BSL 2
- Controlled access
- Hand washing sink
- Sharp hazards warning policy
- Physical containment device
- PPE
- Laboratory bench
- Autoclave
FEATURES OF BSL3
- Self-closing, double-door access
- Controlled access
- Personal shower out
- Sharp hazards warning policy
- Hand washing sink
- Sealed penetrations
- Physical containment device
- Powered air purifying respirator
- Laboratory bench
- Autoclave
- Exhaust HEPA filter
- Effluent decontamination system
- Air tight when disinfecting
FEATURES OF BSL4
- Air tight
- Self-closing, double-door access
- Controlled access
- Sharp hazards warning policy
- Hand washing sink
- Sealed penetrations
- Physical containment device
- Positive pressure protective suit
- Laboratory bench
- Autoclave
- Chemical shower out
- Personal shower out
- Supply and exhaust HEPA filter
- Effluent decontamination system
Most important means of preventing the spread of infection
HAND WASHING
Duration for handwashing accdg. to CDC
20 secs
rinsed downward
Performed if hands are visibly soiled
wash hands with soap and water for 1-2 mins
Performed if hands are NOT visibly soiled
wash hands for at least 30 secs. or
use alcohol-based hand cleaners
Worn to protect clothing and skin
Protect workers from contamination
Laboratory gown
Worn to protect against splashes and inhalation of droplets containing pathogens from infective px.
Masks, goggles, face shields
Worn to protect healthcare workers from contamination by px body substances.
Gloves
Correct sequence of DONNING
- Gown
- Mask (covers both nose & mouth)
- Gloves (pulled over the gown cuff)