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LAB MANAGEMENT (BSC) Flashcards

1
Q

Enclosed workspaces with a ventilated hood designed to contain pathogenic microorganisms during microbiological processes

A

BIOSAFETY CABINETS

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2
Q

Primary purpose of BSC

A

Protect laboratory personnel and the environment from pathogenic microorganism, as aerosols might be formed during the processing of such microorganisms.

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3
Q

T/F
BSC are only used for certain risk group organisms and for processes that might result in aerosol formation.

A

T

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4
Q

decontaminate the air moving out of the cabinet

A

HEPA-filters

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5
Q

Similarity of Laminar hood and BSC

A

Work as enclosed workspaces

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6
Q

Difference of LAMINAR HOOD from BSC

A

Laminar hood: Only protects sample

BSC: Protects sample, personnel, environment

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7
Q

Used BSC for BSL 1 and 2

A

I, II

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8
Q

Used BSC for BSL 3 or 4

A

III

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9
Q

BSC CLASS:

Protection: environment, personnel

A

I

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10
Q

Most basic biosafety cabinet

A

I

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11
Q

Open front. Unsterilized room air enters and passes through High Efficiency Particulate Air (HEPA) filter before being exhausted

A

I

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12
Q

Typically used to either enclose specific equipment like centrifuges or for procedures like aerating cultures that might potentially generate aerosols.

A

I

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13
Q

TYPES OF BSC CLASS I

A

Ducted: connected to the building exhaust system

Unducted: recirculating filtered exhaust back into the laboratory

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14
Q

INFLOW VELOCITY OF BSC CLASS I

A

75

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15
Q
  • Room air is drawn in through the opening that also allows the entry of the operator’s arm during work.
  • Air inside the cabinet then takes in the aerosol particles that may have been generated and moves it away from the operator towards the HEPA filter
  • Air moving out of the cabinet is thus, sterilized via the HEPA filters before its discharge to the environment.
  • Air moves in the front, towards the back, other recirculates within the working area before being exhausted from the HEPA filter at the top of the cabinet. Filtered air stays in the room.
A

CLASS I

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16
Q

AIR EXHAUSTED OF BSC CLASS I

A

100

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17
Q

AIR RECIRCULATED OF BSC CLASS I

A

0

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18
Q

BSC CLASS
Protection: worker, environment, product

A

II

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19
Q

An open-front, ventilated cabinet with vertical laminar flow. The air inside the cabinet is vented through a HEPA filter and recirculated through the cabinet. The exhaust from the cabinet is filtered through a HEPA filter.

A

II

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20
Q

Involves a fan mounted in the top of the cabinet that draws a curtain of sterile air over the workstation where the biological products are being handled.

A

II

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21
Q

Divided into 5 types depending on the: exhaust system and mechanism of work (recirculation of the exhaust air)

A

II

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22
Q

Provides protection to the samples and environment, since make up air is also HEPA-filtered.

A

II

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23
Q

Minimum required class in the laboratories.

A

II

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24
Q
  • Air moves underneath the work station and back up to the top of the cabinet before passing through the HEPA filters.
  • The exhaust that moves out of the facility consists of air being drawn into the front of the cabinet underneath the work surface.
  • Air drawn in acts as a barrier against the potentially contaminated air coming back out to the operator
A

II

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25
Q

Not widely used (not safe to work with hazardous chemical subs.)

A

II – A1

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26
Q

Contaminated air divided just above the work station and mixes with the inflow air

A

II – A1

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27
Q

AIRFLOW VELOCITY OF CLASS II A1

A

75

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28
Q
  • Mixed air is drawn through a duct network so that it reaches the back of the cabinet
  • Air might be either recirculated after passing through the HEPA filters or exhausted out of the cabinet (also through a HEPA filter)
A

II – A1

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29
Q

AIR EXHAUSTED OF CLASS II A1

30
Q

AIR RECIRCULATED OF CLASS II A1

31
Q

Not extensively used due to the chances of release of hazardous chemicals into the environment

32
Q

What is protected in Class II A2 when the air enters the chamber through front aperture

A

operator/personnel

33
Q

If hazardous, volatile chemicals are to be used within the cabinet, along with the microbiological work, exhaust must be released into the atmosphere through the direct duct system

34
Q

Inflow velocity of II – A2

35
Q

Inflow air mixes with the downflow air (from the top of the cabinet) and enters the front intake grille and then passes over the workstation where the air splits.

36
Q

60-70% of the contaminated air: recycled and pushed back into the workstation in the chamber through the downflow HEPA filter
30-40% of the contaminated air: exhausted through the exhaust HEPA filter

37
Q

Air exhausted by II A2

38
Q

Air recirculated by II A2

39
Q

uses single-pass airflow to control the flow of hazardous vapors

A

Type B cabinets
(difference from type A)

40
Q

Has dedicated duct system which allows the release of the contaminated air out of the facility.

41
Q

Has split downflow air – prevent buildup of chemicals within the cabinet.

42
Q

Allows handling of volatile chemicals.

43
Q

Inflow velocity of II B1

44
Q

Divide airflow so that the contaminated air is directed towards the exhaust system while the air between the operator and the workstation mixes with the inflow and is recirculated.

Exhaust air dispersed out of the facility should be passed through the HEPA filters to provide protection to the environment.

45
Q

Air exhausted by II-B1

46
Q

Air recirculated by II-B1

47
Q

aka “Total exhaust cabinet”

48
Q

Similarity to Type A: air is drawn in from the front opening creating an air barrier that protects the operator

49
Q

Advantage: removal of toxic vapors generated in the cabinet with no recirculation within the BSC

Best to be used for tasks involving chemical vapor release

For toxic and radionucleotides

50
Q

Disadvantage: Expensive; limited to toxicology laboratories where protection against hazardous chemicals is imperative

51
Q

Inflow velocity of II - B2

52
Q
  • Air is drawn in from an opening at the top of the cabinet that supplies the downflow of air in the cabinet.
  • The air then passes through a HEPA filter, where 100% of the air is exhausted through a dedicated duct system with an exhaust fan motor. The air moving out of the facility is thus sterilized before its release into the atmosphere.
  • All of the contaminated airflow (100%) is externally exhausted which means the air drawn into the cabinet is 100% exhausted into the atmosphere. None of the air drawn into the BSC from either inflow or downflow is recycled within the airflow system (only passes once, no recirculation)
53
Q

Air exhausted by II - B2

54
Q

Air recirculated by II - B2

55
Q

Similarity to Type B: working mechanism (however, these are designed to reduce operating costs & add flexibility to the laboratories)

56
Q

Difference of Type C from Type A

A

Type C uses a single-pass airflow mechanism where air is not circulated.

57
Q

Difference of Type C from Type B

A

Type C
• do not require a dedicated ducted exhaust system
• can work for an extended duration to increase operator protection in the case of exhaust failure
• run without the exhaust at all

58
Q

Inflow velocity of II - C1

59
Q
  • Uses single-pass airflow system where cabinets move the air by mixing it with the downflow air separated into columns for recirculation.
  • Air above the workstation is drawn with a second fan which moves the contaminated air out through the exhaust system with a HEPA filter
60
Q

Air exhausted by II - C1

61
Q

Air recirculated by II - C1

62
Q

Provide maximum protection to the operator, the environment, and the sample against high
-risk group 4 pathogenic organisms

63
Q

A totally enclosed, ventilated cabinet. Laboratory workers manipulate items in the cabinet through attached rubber gloves that are accessible from outside the cabinet (this is why these cabinets are also termed ‘GLOVE BOXES’)

64
Q

Leak-tight, totally enclosed but ventilated cabinets, where all air that either enters or leaves through the facility pass through a HEPA filter

65
Q

Can be used for all four BSL (1, 2, 3, 4) but most important for manipulation of biological materials in the BSL
4 (i.e. Ebola virus)

66
Q

Mostly custom-built for specific laboratories with lab equipment build inside the chamber

67
Q

Has a transfer chamber that facilitates the sterilization of materials before they leave the glove box

68
Q

Provided with rubber gloves that are attached to the system to be
used during operations in the cabinet.

69
Q

Inflow velocity of III

70
Q

Intake air is filtered through HEPA filters, and the exhaust air is filtered/treated through: double HEPA filters (arranged in a series) or HEPA filters in combination with incineration before being discharged outside of the building

71
Q

Air exhausted by BSC CLASS III

72
Q

Air recirculated by BSC CLASS III

MTAP 2 (23 decks)

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