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MTAP 2 > Hema (Stains) > Flashcards

Hema (Stains) Flashcards

1
Q

Stains ALP in neutrophil and to a small degree, in certain B cells.

A

Leukocyte Alkaline
Phosphatase

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1
Q

Stains peroxidases present in granulocytes and monocytes

A

Peroxidase

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2
Q

Stains lipids in monocytes and granulocytes

A

Sudan Black B

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3
Q

Stains esterases present in monocytic cells, macrophages,
megakaryocytes and platelets

A

Non-specific esterase

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4
Q

Stains esterases present in granulocytes

A

Chloroacetate
esterase

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5
Q

Stain mucoproteins, glycoproteins, and high molecular weight
carbohydrates normally present in almost all blood types except
pronormoblast

A

Periodic Acid Schiff

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6
Q

Diagnose Di Guglielmo’s syndrome and aid to classify some
acute leukemias

A

PAS

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7
Q

Differentiated by LAP

A

Chronic myelogenous from
leukemoid reaction or PV

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7
Q

Stains ACP present in the myelogenous cell, lymphocytes,
plasma cells, monocytes and platelets
L (+) tartaric acid – diagnose hairy cell leukemia

A

Acid phosphatase

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8
Q

Differentiated by Peroxidase

A

Acute myelogenous and monocytic leukemia from
Acute lymphocytic leukemia

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9
Q

Differentiated by SBB

A

Acute myelogenous and myelomonocytic leukemias from
Acute lymphocytic leukemia

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9
Q

Differentiated by Chloroacetate esterase

A

Granulocytic cells from
Monocytic cells

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10
Q

Differentiated by NSE

A

Monocytic leukemias from
Granulocytic leukemias

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10
Q

Non-enzyme stains

A

Sudan Black B
PAS
Toluidine blue

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11
Q

Marker for primary granules of
Auer rods

A

MPO

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12
Q

Marker for phospholipids and lipids

A

Sudan Black B

13
Q

Stains DNA polymerase
immunoperoxidase

A

Terminal
deoxyribonucleotidyl
transferase (TdT)

14
Q

Marker for glycogen, glycoproteins,
mucoproteins and high molecular
weight carbohydrates

A

PAS

15
Q

Marker for monocyte,
megakaryocyte, and plasma cells

A

Alpha-naphthyl
acetate esterase

15
Q

Marker for mature and immature
neutrophils and mast cells

A

Naphthol AS-D
chloroacetate
esterase

16
Q

Identifying monocytes,
promonocytes, and monoblasts

A

Alpha- naphthyl
Butyrate esterase

17
Q

Present in all hematopoietic cells
and found on lysosomes

A

Acid phosphatase

18
Q

Marker for hairy cell leukemia

A

Tartrate resistant
acid phosphatase

19
Q

Neutrophils – only leukocyte that
contains this activity

A

Leukocyte alkaline
Phosphatase (LAP)

20
Q

Binds with acid
mucopolysaccharides in blood cells

A

Toluidine Blue

21
Q

Present in 90% cases of ALL

A

TdT

22
Q

Peroxidase activity produces dark brown
granules in cytoplasm of granulocytes and
monocytes

A

MPO

23
Q

Dark purple black granules in neutrophil
precursors
Lymphoblasts are negative

A

SBB

24
Q

Activity results in bright fuchsia pink or
magenta red Pattern of staining varies with
each cell type

A

PAS

25
Q

Enzyme activity results in bright red granules in cytoplasm of neutrophils, neutrophil precursors and mast cells

A

Naphthol AS-D chloroacetate esterase

26
Q

Monocytic stain red-brown

A

Alpha-naphthyl
acetate esterase

27
Q

Enzyme activity results in dark red
precipitates in cytoplasm

A

Alpha- naphthyl
Butyrate esterase

28
Q

Activity is indicated by purple to red granules

A

Acid phosphatase

29
Q

Activity is indicated by purple to dark red
granules in cytoplasm

A

Tartrate resistant
acid phosphatase

30
Q

100 cell count is done.
Neutrophils are scored from with no activity
to 4 with a large amount of activity

A

LAP

31
Q

Strongly metachromatic

A

Toluidine Blue

32
Q

nonspecific esterases

A

Alpha-naphthyl
acetate esterase

Alpha- naphthyl
Butyrate esterase

MTAP 2 (23 decks)

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