Hema (Stains) Flashcards
Stains ALP in neutrophil and to a small degree, in certain B cells.
Leukocyte Alkaline
Phosphatase
Stains peroxidases present in granulocytes and monocytes
Peroxidase
Stains lipids in monocytes and granulocytes
Sudan Black B
Stains esterases present in monocytic cells, macrophages,
megakaryocytes and platelets
Non-specific esterase
Stains esterases present in granulocytes
Chloroacetate
esterase
Stain mucoproteins, glycoproteins, and high molecular weight
carbohydrates normally present in almost all blood types except
pronormoblast
Periodic Acid Schiff
Diagnose Di Guglielmo’s syndrome and aid to classify some
acute leukemias
PAS
Differentiated by LAP
Chronic myelogenous from
leukemoid reaction or PV
Stains ACP present in the myelogenous cell, lymphocytes,
plasma cells, monocytes and platelets
L (+) tartaric acid – diagnose hairy cell leukemia
Acid phosphatase
Differentiated by Peroxidase
Acute myelogenous and monocytic leukemia from
Acute lymphocytic leukemia
Differentiated by SBB
Acute myelogenous and myelomonocytic leukemias from
Acute lymphocytic leukemia
Differentiated by Chloroacetate esterase
Granulocytic cells from
Monocytic cells
Differentiated by NSE
Monocytic leukemias from
Granulocytic leukemias
Non-enzyme stains
Sudan Black B
PAS
Toluidine blue
Marker for primary granules of
Auer rods
MPO
Marker for phospholipids and lipids
Sudan Black B
Stains DNA polymerase
immunoperoxidase
Terminal
deoxyribonucleotidyl
transferase (TdT)
Marker for glycogen, glycoproteins,
mucoproteins and high molecular
weight carbohydrates
PAS
Marker for monocyte,
megakaryocyte, and plasma cells
Alpha-naphthyl
acetate esterase
Marker for mature and immature
neutrophils and mast cells
Naphthol AS-D
chloroacetate
esterase
Identifying monocytes,
promonocytes, and monoblasts
Alpha- naphthyl
Butyrate esterase
Present in all hematopoietic cells
and found on lysosomes
Acid phosphatase
Marker for hairy cell leukemia
Tartrate resistant
acid phosphatase
Neutrophils – only leukocyte that
contains this activity
Leukocyte alkaline
Phosphatase (LAP)
Binds with acid
mucopolysaccharides in blood cells
Toluidine Blue
Present in 90% cases of ALL
TdT
Peroxidase activity produces dark brown
granules in cytoplasm of granulocytes and
monocytes
MPO
Dark purple black granules in neutrophil
precursors
Lymphoblasts are negative
SBB
Activity results in bright fuchsia pink or
magenta red Pattern of staining varies with
each cell type
PAS
Enzyme activity results in bright red granules in cytoplasm of neutrophils, neutrophil precursors and mast cells
Naphthol AS-D chloroacetate esterase
Monocytic stain red-brown
Alpha-naphthyl
acetate esterase
Enzyme activity results in dark red
precipitates in cytoplasm
Alpha- naphthyl
Butyrate esterase
Activity is indicated by purple to red granules
Acid phosphatase
Activity is indicated by purple to dark red
granules in cytoplasm
Tartrate resistant
acid phosphatase
100 cell count is done.
Neutrophils are scored from with no activity
to 4 with a large amount of activity
LAP
Strongly metachromatic
Toluidine Blue
nonspecific esterases
Alpha-naphthyl
acetate esterase
Alpha- naphthyl
Butyrate esterase
