Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

From Flashcardlet > Lab Final > Flashcards

Lab Final Flashcards

1
Q

In Citrate medium, what is the:
Nitrogen Source
Carbon

A

N=ammonium phosphate

C=sodium citrate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Bacteria must possess ________ to convert molecules into pyruvate.

A

citrate-permease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

color and texture of citrate slant inoculated

A

Green

solid agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Citrate test result is blue means?

A

Citrate is utilized
PH increases
test is +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Citrate color is Green and shows growth means?

A

Citrate is utilized
Inoculation not complete
test is +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Citrate color is Green and shows NO growth means?

A

Citrate is NOT utilized

Test is -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Malonate and citrate tests are associated with what chain of events

A

Kreb Cycle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Important enzyme in malonate test

A

Succinate dehydrogenaze

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Important chemical reduction in malonate test

A

FADH—>FADH2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Regular color and texture of molonate broth

A

green liquid broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

If malonate broth is dark blue it means?

A

Malonate Utilized

Test is +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

If Malonate broth has no color change or slightly yellow

A

No Malonate Utilized

Test is -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Control color and texture of Phenylalanine Deaminase test

A

Yellowish Goo

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

If Phenylalanine Deaminase test is green it means?

A

Phenylalanine Deaminase is present

Test is +

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

If Phenylalanine Deaminase test is Yellow it means?

A

Phenylalanine Deaminase is absent

Test is -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Organisms that produce Phenylalanine Deaminase remove ________ from Phenylalanine?

A

amino group

17
Q

Reagent used in Phenylalanine Deaminase Test

A

FeCl3

Ferric Chloride

18
Q

Product of Phenylalanine Deaminase reaction with FeCl3

A

Phenylpyruvic Acid

19
Q

Reactions that use water to split comlex molecules

A

hydrolysis

19
Q

What reagent is used in Starch Hydrolysis to help detect the presence or absence of starch

A

Iodine

20
Q

Type and names of enzymes associated with starch hydrolysis

A

Extracellular

amylase & oligo-1,6 glucosidase

21
Q

how do enzymes hydrolyze starch

A

breaking the glycosidic linkages between sugar subunits

22
Q

End result of starch hydrolysis

A

Breaking down polysacharides into smaller units

23
Q

In the starch hydrolysis test, what does a clearing around the growth indicate?

A

Amylase is present

Test is +

24
Q

In the starch hydrolysis test, what doe NO clearing around the growth indicate?

A

No amylase is present

Test is Negative(-)

25
Q

How do you inoculate the SIM test

A

stab the Butt!!!

26
Q

S in the SIM stands for?

A

sulfur

Black Precipitate

27
Q

I in SIM stands for ?

A

Indole

28
Q

In the SIM test, what does a black precipitate indicate

A

Sulfur Reduction

Test is +

29
Q

In the Indole SIM test, What does a thin red line in the alcohol layer of Kovac’s reagent mean and what test does this represent?

A

Presence of tryptophanase

Test is +

30
Q

What reagent is added in the SIM (indole)test

A

Kovac’s Reagent

31
Q

Enzyme in SIM test that catalyzes the putrefaction of amino acid cysteine to pyruvate.

A

Cysteine Desulfurase

32
Q

Enzyme in SIM test that catalyzes reduction of sulfur in form of sulfate at the end of electron transport chain

A

Thiosulfate Reductase

33
Q

Both Chemical reactions involved in the SIM test produce what gas?

A

H2S

Hydrogen Sulfide Gas

34
Q

What does the M in SIM test stand for?

A

Motility

35
Q

In the Motility test, what is a positive result?

A

Growth radiating in all directions

Slightly Fuzzy

36
Q

In the Motility test, what is considered a negative test result?

A

No radiating Growth

Except a little in the stab area

From Flashcardlet (67 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions