Lab Exam

Question 1

enzymes

Answer 1

biological catalyst that increase the rates of biochemical reactions in cells by facilitating the conversion of a specific substrate to a product by lowering the energy of activations

Question 2

The catalytic effect of an enzyme is determined by its

Answer 2

shape, particularly the shape of its active site which will bind only with certain substrate

Question 3

How can enzyme activity be determined?

Answer 3

By measuring the decrease in substrate or increase in product

Question 4

How does a spectrophotometer work? (4)n

Answer 4

1. white light seoerated into different wavelengths by a prism
2. slit allows only one specific wavelength of light selected by user
3. light wavelength selected will pass through the sample solution. Some light absorbed white some transmitted (the higher concentration of coloured pigment or indicator in t=solution the greater absorbance
4. The transmitted light strikes the photocell that converts light energy to an electric current measured by a meter.

Question 5

we wipe the covette bc

Answer 5

the moisture and fingerprint can scatter or absorb light

Question 6

v,max

Answer 6

maximum rate an enzyme can catalusze the reaction. All enzyme molecules are complexed with the subtle and adding any additional substrate will not speed up the reaction

Question 7

Km

Answer 7

the substrate concentration at which the reaction proceeds at half of its maximum rate

1/2 max