Lab 4

Question 0

Define contaminants

Answer 0

Unwanted microbes

Question 1

Define innoculated

Answer 1

Introduced into environment

Question 2

Define aseptic technique

Answer 2

Technique to exclude contaminants

Question 3

Define steralized

Answer 3

Kills all bacteria

Question 4

Define broth cultures

Answer 4

Large number of bacteria, easy to transport

Question 5

Define agar slants

Answer 5

Good growth surface, easy to store and transport

Question 6

Define agar deep

Answer 6

Used to grow bacteria who need less oxygen, also used to check motility

Question 7

What is an innoculating loop

Answer 7

Loop used to inoculate

Question 8

What is an inoculating needle

Answer 8

Needle used to inoculate agar deeps

Question 9

What is an agar slant inoculation

Answer 9

Inoculate a slant by streaking the loop back and forth on the surface of the agar

Question 10

What is agar deep innoculation

Answer 10

Innoculate an agar deep by stabbing into the agar with a needle

Question 11

How do you inoculate a broth

Answer 11

Inoculate a broth by taking a piece of the bacteria and put it into the liquid

Question 12

Why should the entire wire of the inoculating loop be red

Answer 12

For disinfection

Question 13

Why don’t we set down the cap

Answer 13

Contamination

Question 14

How many times do we flame the opening of the tube

Answer 14

3

Question 15

Why must the loop be cooled before you pick up bacteria

Answer 15

So that you don’t kill them and cause an aerosol

Question 16

What in the broth signals growth of bacteria

Answer 16

Turbidity

Question 17

Which bacteria were gram positive

Answer 17

S. Aureus

Question 18

Which bacteria were gram negative

Answer 18

E. Coli