Lab 5

Question 0

What is fixing

Answer 0

Kills bacteria. Coagulated proteins from the cells will cause the cells to stick to the slide

Question 1

What is a smear

Answer 1

Thin film of bacteria cells

Question 2

What is heat fixing

Answer 2

Use heat to stick cells to slide

Question 3

What is chemical fixing

Answer 3

Put methanol on cells to fix it to the slide

Question 4

What is a chromophore

Answer 4

Colored ion

Question 5

What charge does a basic stain have

Answer 5

Positive

Question 6

What charge does an acidic stain have

Answer 6

Negative

Question 7

Define simple stain

Answer 7

Only one stain

Question 8

Define direct stain

Answer 8

Stains the bacteria

Question 9

Define negative stain

Answer 9

Stains the background

Question 10

Which stain was used in this lab

Answer 10

Methylene blue

Question 11

What bacteria are used in thiS lab

Answer 11

S. Epidermidis

B. Megaterium

Question 12

Describe the procedure of the simple stain from a solid medium

Answer 12

place one loop fulls of water on the slide. Transfer a very small amount of the culture with the sterile loop. Mix with the water on the slide.pass the slide under the flame of a burner three times. Cover the spear with methylene blue and leave it for 30 seconds. Wash off methylene blue. Blot it dry.

Question 13

Describe the procedure of a simple stained from a liquid medium

Answer 13

Place 2 loop fulls of the Liquid culture on the slide with the sterile loop. Spread the bacteria within the circle. Pass the slide through flame three times. Cover the smear with methylene blue and leave it for 30 seconds. Wash off methylene blue, blot it dry.

Question 14

What happens if you apply too much heat

Answer 14

It distorts the cells