lab 3 - enzymes II Flashcards
what is Vmax
max rate at which an enzyme can catalyse the reaction under the conditions of the experiment
what happens at Vmax
all enzyme molecules are complexed with substrate molecules and adding any additional substrate will NOT speed up the reaction
what is Km
the substrate concen at which the reaction proceeds at its max catalytic efficiency
determined by reading the substrate concen at half of its max rate
what is a limitation of a michaelis menten plot
Vmax is an asymptote
value can only be determined if the reaction is run at high enough concens of substrate
what is a lineweaver burk plot
inverse of michaelis menten
slope of the line = Km/Vmax
y intercept = 1/Vmax
x intercept = -1/Km
more precise than michaelis menten
what are competitive inhibitors
similar in shape to enzyme substrate and compete with the substrate for binding to the active site
depends on the relative concen of substrate and inhibitor
what is the effect of competitive inhibition on Vmax and Km
Vmax = unchanged
Km = increases
what are non competitive inhibitors
don’t compete with the substrate for the active site but bind to other sites on the enzyme
makes the active site less efficient and reduces the rate even at high concen of substrate
what is the effect of non competitive inhibition on Vmax and Km
Vmax = reduced
Km = unchanged