lab 3 - enzymes II Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

what is Vmax

A

max rate at which an enzyme can catalyse the reaction under the conditions of the experiment

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what happens at Vmax

A

all enzyme molecules are complexed with substrate molecules and adding any additional substrate will NOT speed up the reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what is Km

A

the substrate concen at which the reaction proceeds at its max catalytic efficiency

determined by reading the substrate concen at half of its max rate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what is a limitation of a michaelis menten plot

A

Vmax is an asymptote
value can only be determined if the reaction is run at high enough concens of substrate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what is a lineweaver burk plot

A

inverse of michaelis menten

slope of the line = Km/Vmax
y intercept = 1/Vmax
x intercept = -1/Km

more precise than michaelis menten

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what are competitive inhibitors

A

similar in shape to enzyme substrate and compete with the substrate for binding to the active site

depends on the relative concen of substrate and inhibitor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what is the effect of competitive inhibition on Vmax and Km

A

Vmax = unchanged
Km = increases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what are non competitive inhibitors

A

don’t compete with the substrate for the active site but bind to other sites on the enzyme

makes the active site less efficient and reduces the rate even at high concen of substrate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what is the effect of non competitive inhibition on Vmax and Km

A

Vmax = reduced
Km = unchanged

How well did you know this?
1
Not at all
2
3
4
5
Perfectly