lab 0 - general info Flashcards
what are the magnifying components of the microscope
ocular and objective lens systems
what is the condenser lens
gathers and focuses light onto the specimen
light rays pass directly from the light source onto the condenser
where is the iris diaphragm
located directly beneath the condenser
what is the function of the iris diaphragm
regulates the amount of light entering the condenser
what is the function of the fine and coarse adjustment knobs
focus on the specimen
what is the ocular division equal to under 10x objective
10 micrometres
what is the ocular division equal to under 40x objective
2.5 micrometres
what is the ocular division equal to under 100x objective
1 micrometre
what is the magnification of the ocular lens
10x
what is the total magnification with an ocular mag of 10x and objective of 4x
40x
what is the total magnification with an ocular mag of 10x and objective of 10x
100x
what is the total magnification with an ocular mag of 10x and objective of 40x
400x
what is the total magnification with an ocular mag of 10x and objective of 100x
1000x
what type of lenses are in the microscope
parfocal - can switch between lenses with only small adjustments in the fine adjustment knob
what is the association between depth of field and field of view
field of view gets smaller - depth of field decreases
thinner layer of specimen will be in focus when you use higher magnification
