Lab 3 Flashcards
Deoxyribonucleic acid (DNA)
Large organic molecules composed of two chains of nucleic acids
What is DNA’s three-dimensional structure?
Double helix twisted in the clockwise direction; resembles a spiral ladder
Nucleotides
Many individual molecules in a nucleic acid molecule; linked by covalent bonds between their sugar phosphate groups to form the nucleic strand
Three subunits of nucleotides
Phosphate group, deoxyribose sugar (five-carbon sugar), and one of four nitrogenous bases
Two categories of nitrogenous bases
Purines and pyrimidines
Purines
Composed of two nitrogen containing rings and two nitrogenous bases called adenine and guanine
Pyrimidines
Have one nitrogen containing ring and 2 nitrogenous bases called thymine and cytosine
Complementary base pairing
A-T, G-C
Template (nucleic acid)
Is a part or model which serves as a guide for the production of an oppositely shaped structure
What enzyme does DNA replication begin?
DNA helicase
DNA helicase
Temporarily “unzips” the hydrogen binds between the purine and pyrimidines bases in a short segment of DNA
What is the DNA replication process?
DNA helicase unzips the hydrogen bonds between the bases then the nucleotides move into place in the process of complementary base-pairing. DNA polymerase (enzyme) joins the nucleotides by pairing the sugar-phosphate groups to form a new complementary nucleic acid strand.
Why is DNA replication called “semi conservative?”
Because each new molecule results from one old nucleic strand and one new nucleic acid strand
Exonuclease
Enzyme that controls accuracy of DNA replication and prevents the incorporation of mismatched nucleotides into a new nucleic acid strand by removing any mismatched nucleotides from the strand and replaces it with appropriate nucleotide
Mutation
Mistakes that occur during replication and are very important to population because they create genetic variability