Lab 1 Flashcards
Ocular lens
“Eye piece”; part of the microscope that is looked through
Objective lenses
Attached to a nosepiece or turret at the base of the microscope’s body tube
How do you calculate total magnification?
Ocular lens x objective lens
Body tube
Transfers light from the objective lenses through another series of lenses to the ocular lens
Arm
Connects the body tube to the rest of the instrument
Stage
Platform on which the microscope slide rests
Diaphragm
One of the controls that varies the amount of light reaching a specimen
Condenser
Focuses light onto the specimen
Light source
Illuminates the specimen on stage
Coarse adjustment
Bring a specimen initially into focus; usually large knobs located on the sides of the arm, just below the microscope’s stage
Fine adjustment
Used for small adjustments in focus
How to prepare a wet mound
- Obtain a clean microscope slide and a cover slip.
- Use the broad end of the toothpick to scrape the inside of your cheek.
- Place the cells on the center of the slide.
- Place a water drop on the cells and stir the cells with the toothpick.
- Allow the cells to dry for a minute or two.
- Place the cover slip next to the water drop at about a 60 degree angle.
- Slowly lower the cover slip until it rests flat on the slide.
- Place a drop or two of METHYLENE-BLUE stain on the slide beside the cover slip.
- Use a piece of paper towel to draw the stain under the cov slip by placing it opposite the stain.
Dorsal
Towards the back of the body or the vertebral column; ex: the vertebral column is dorsal to the lungs
Ventral
Towards the front of the body; ex: the sternum is central to the lungs
Anterior
2 legged: toward the front of the body or the ventral surface; 4 legged: towards the head; ex: the sternum is anterior to the vertebrae
Posterior
2 legged: towards the body or dorsal surface of the body; 4 legged: toward the tail; ex: the lungs are posterior to the sternum
Proximal
Closer to the point of reference; ex: the femoral head is on the proximal end of the leg
Distal
Further from the point of reference; ex: the foot is on the distal end of the leg
Superior
Above the point of reference; ex: the eyes are superior to the nose
Inferior
Below the point of reference; ex: the mouth is inferior to the eyes
Superficial
Towards the surface of the body; ex: the skin is superficial
Deep
Away from the body’s surface; ex: the heart is deep
Medial
Towards the midline or the median plane of the body; ex: the sternum is medial
Lateral
Away from the midline or the median plane of the body; ex: the ears are lateral
